Robert J Brooker

2013

Scale-up in a large introductory biology course

Brooker, R., Matthes, D., Wright, R., Wassenberg, D., Wick, S. & Couch, B., Jan 1 2013, Connected Science: Strategies for Integrative Learning in College. Indiana University Press, p. 89-99 11 p.

Research output: Chapter in Book/Report/Conference proceeding › Chapter

2012

A facile transport assay for H ⁺ coupled membrane transport using fluorescence probes

Lan, W., Ren, H., Pang, Y., Huang, C., Xu, Y., Brooker, R. J. & Zhang, J., Jan 1 2012, In: Analytical Methods. 4, 1, p. 44-46 3 p.

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

2010

Evidence that highly conserved residues of transmembrane segment 6 of escherichia coli mnth are important for transport activity

Haemig, H. A. H., Moen, P. J. & Brooker, R. J., Jun 8 2010, In: Biochemistry. 49, 22, p. 4662-4671 10 p.

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

2006

A suppressor analysis of residues involved in cation transport in the lactose permease: Identification of a coupling sensor

Franco, P. J., Matzke, E. A., Johnson, J. L., Wiczer, B. M. & Brooker, R. J., May 1 2006, In: Journal of Membrane Biology. 211, 2, p. 101-113 13 p.

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

2004

Control of H⁺/lactose coupling by ionic interactions in the lactose permease of escherichia coli

Johnson, J. L. & Brooker, R. J., Apr 1 2004, In: Journal of Membrane Biology. 198, 3, p. 135-146 12 p.

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Importance of conserved acidic residues in MntH, the Nramp homolog of Escherichia coli

Haemig, H. A. H. & Brooker, R. J., Nov 2004, In: Journal of Membrane Biology. 201, 2, p. 97-107 11 p.

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

2003

Evidence for structural symmetry and functional asymmetry in the lactose permease of Escherichia coli

Green, A. L., Hrodey, H. A. & Brooker, R. J., Sep 30 2003, In: Biochemistry. 42, 38, p. 11226-11233 8 p.

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Role of glutamate-126 and arginine-144 in the lactose permease of Escherichia coli

Johnson, J. L. & Brooker, R. J., Feb 4 2003, In: Biochemistry. 42, 4, p. 1095-1100 6 p.

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

2002

An isotope-edited FT-IR study of a symporter, the lactose permease

Patzlaff, J. S., Zhang, J., Brooker, R. J. & Barry, B. A., Jun 11 2002, In: Biochemistry. 41, 23, p. 7366-7372 7 p.

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

2001

A face on transmembrane segment 8 of the lactose permease is important for transport activity

Green, A. L. & Brooker, R. J., Oct 9 2001, In: Biochemistry. 40, 40, p. 12220-12229 10 p.

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

A triple mutant, K319N/H322Q/E325Q, of the lactose permease cotransports H⁺ with thiodigalactoside

Johnson, J. L., Lockheart, M. S. K. & Brooker, R. J., Jun 1 2001, In: Journal of Membrane Biology. 181, 3, p. 215-224 10 p.

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

2000

A reaction-induced fourier transform-infrared spectroscopic study of the lactose permease. A transmembrane potential perturbs carboxylic acid residues

Patzlaff, J. S., Brooker, R. J. & Barry, B. A., Sep 15 2000, In: Journal of Biological Chemistry. 275, 37, p. 28695-28700 6 p.

Research output: Contribution to journal › Article › peer-review

Open Access

3 Scopus citations

A revised model for the structure and function of the lactose permease: Evidence that a face on transmembrane segment 2 is important for conformational changes

Green, A. L., Anderson, E. J. & Brooker, R. J., Jul 28 2000, In: Journal of Biological Chemistry. 275, 30, p. 23240-23246 7 p.

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Roles of charged residues in the conserved motif, G-X-X-X-D/E-R/K-X-G- [X]-R/K-R/K, of the lactose permease of Escherichia coli

Pazdernik, N. J., Matzke, E. A., Jessen-Marshall, A. E. & Brooker, R. J., 2000, In: Journal of Membrane Biology. 174, 1, p. 31-40 10 p.

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

The conserved motif in hydrophilic loop 2/3 and loop 8/9 of the lactose permease of Escherichia coli. Analysis of suppressor mutations

Cain, S. M., Matzke, E. A. & Brooker, R. J., Aug 19 2000, In: Journal of Membrane Biology. 176, 2, p. 159-168 10 p.

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

1999

A K319N/E325Q double mutant of the lactose permease cotransports H⁺ with lactose: Implications for a proposed mechanism of H⁺/lactose symport

Johnson, J. L. & Brooker, R. J., Feb 12 1999, In: Journal of Biological Chemistry. 274, 7, p. 4074-4081 8 p.

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

1998

Fourier transform infrared analysis of purified lactose permease: A monodisperse lactose permease preparation is stably folded, α-helical, and highly accessible to deuterium exchange

Patzlaff, J. S., Moeller, J. A., Barry, B. A. & Brooker, R. J., Nov 3 1998, In: Biochemistry. 37, 44, p. 15363-15375 13 p.

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

1997

An analysis of suppressor mutations suggests that the two halves of the lactose permease function in a symmetrical manner

Pazdernik, N. J., Cain, S. M. & Brooker, R. J., Oct 17 1997, In: Journal of Biological Chemistry. 272, 42, p. 26110-26116 7 p.

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Lactose carrier mutants of Escherichia coli with changes in sugar recognition (Lactose versus melibiose)

Varela, M. F., Brooker, R. J. & Wilson, T. H., Sep 1997, In: Journal of bacteriology. 179, 17, p. 5570-5573 4 p.

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Role of conserved residues in hydrophilic loop 8-9 of the lactose permease

Pazdernik, N. J., Jessen-Marshall, A. E. & Brooker, R. J., 1997, In: Journal of bacteriology. 179, 3, p. 735-741 7 p.

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Suppressor analysis of mutations in the loop 2-3 motif of lactose permease: Evidence that glycine-64 is an important residue for conformational changes

Jessen-Marshall, A. E., Parker, N. J. & Brooker, R. J., 1997, In: Journal of bacteriology. 179, 8, p. 2616-2622 7 p.

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

1996

Evidence that transmembrane segment 2 of the lactose permease is part of a conformationally sensitive interface between the two halves of the protein

Jessen-Marshall, A. E. & Brooker, R. J., Jan 19 1996, In: Journal of Biological Chemistry. 271, 3, p. 1400-1404 5 p.

Research output: Contribution to journal › Article › peer-review

Open Access

43 Scopus citations

Evidence that transmembrane segment 2 of the lactose permease is part of a conformationally sensitive interface between the two halves of the protein

Jessen-Marshall, A. E. & Brooker, R. J., Jan 19 1996, In: Journal of Biological Chemistry. 271, 3, p. 1400-1404 5 p.

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Structural topology of transmembrane helix 10 in the lactose permease of Escherichia coli

Goswitz, V. C., Matzke, E. A., Taylor, M. R., Jessen-Marshall, A. E. & Brooker, R. J., 1996, In: Journal of Biological Chemistry. 271, 36, p. 21927-21932 6 p.

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

1995

Structural features of the uniporter/symporter/antiporter superfamily

Goswitz, V. C. & Brooker, R. J., Mar 1995, In: Protein Science. 4, 3, p. 534-537 4 p.

Research output: Contribution to journal › Article › peer-review

84 Scopus citations

The conserved motif, GXXX(D/E)(R/K)XG[X](R/K)(R/K), in hydrophilic loop 2/3 of the lactose permease

Jessen-Marshall, A. E., Paul, N. J. & Brooker, R. J., Jul 7 1995, In: Journal of Biological Chemistry. 270, 27, p. 16251-16257 7 p.

Research output: Contribution to journal › Article › peer-review

83 Scopus citations

1994

Functional roles of Glu-269 and Glu-325 within the lactose permease of Escherichia coli

Franco, P. J. & Brooker, R. J., Mar 11 1994, In: Journal of Biological Chemistry. 269, 10, p. 7379-7386 8 p.

Research output: Contribution to journal › Article › peer-review

78 Scopus citations

1993

Isolation of lactose permease mutants which recognize arabinose

Goswitz, V. C. & Brooker, R. J., 1993, In: Molecular Membrane Biology. 10, 1, p. 61-70 10 p.

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Lactose permease mutants which transport (malto-)oligosaccharides

Olsen, S. G., Greene, K. M. & Brooker, R. J., 1993, In: Journal of bacteriology. 175, 19, p. 6269-6275 7 p.

Research output: Contribution to journal › Article › peer-review

Open Access

21 Scopus citations

Lactose permease mutants which transport (malto-)oligosaccharides

Olsen, S. G., Greene, K. M. & Brooker, R. J., 1993, In: Journal of bacteriology. 175, 19, p. 6269-6275 7 p.

Research output: Contribution to journal › Article › peer-review

Open Access

21 Scopus citations

1992

An analysis of the side chain requirement at position 177 within the lactose permease which confers the ability to recognize maltose

Gram, C. D. & Brooker, R. J., Feb 25 1992, In: Journal of Biological Chemistry. 267, 6, p. 3841-3846 6 p.

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Functional role of arginine 302 within the lactose permease of Escherichia coli

Matzke, E. A., Stephenson, L. J. & Brooker, R. J., Sep 25 1992, In: Journal of Biological Chemistry. 267, 27, p. 19095-19100 6 p.

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

1991

An analysis of lactose permease "sugar specificity" mutations which also affect the coupling between proton and lactose transport I. VAL¹⁷⁷ and VAL¹⁷⁷/ASN³¹⁹ permeases facilitate proton uniport and sugar uniport

Brooker, R. J., Mar 5 1991, In: Journal of Biological Chemistry. 266, 7, p. 4131-4138 8 p.

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

An analysis of lactose permease "sugar specificity" mutations which also affect the coupling between proton and lactose transport II. Second site revertants of the thiodigalactoside-dependent proton leak by the VAL¹⁷⁷/ASN³¹⁹ permease

Eelkema, J. A., O'Donnell, M. A. & Brooker, R. J., Mar 5 1991, In: Journal of Biological Chemistry. 266, 7, p. 4139-4144 6 p.

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Evidence that the asparagine, 322 mutant of the lactose permease transports protons and lactose with a normal stoichiometry and accumulates lactose against a concentration gradient

Franco, P. J. & Brooker, R. J., 1991, In: Journal of Biological Chemistry. 266, 11, p. 6693-6699 7 p.

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

1990

Characterization of the double mutant, Val-177/Asn-322, of the lactose permease

Brooker, R. J., Mar 5 1990, In: Journal of Biological Chemistry. 265, 7, p. 4155-4160 6 p.

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

The lactose permease of Escherichia coli

Brooker, R. J., 1990, In: Research in Microbiology. 141, 3, p. 309-315 7 p.

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

1989

Analysis of the structural specificity of the lactose permease toward sugars

Olsen, S. G. & Brooker, R. J., 1989, In: Journal of Biological Chemistry. 264, 27, p. 15982-15987 6 p.

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Characterization and sequencing of the lac Y^54-41 'uncoupled' mutant of the lactose permease

Brooker, R. J., Myster, S. H. & Wilson, T. H., 1989, In: Journal of Biological Chemistry. 264, 14, p. 8135-8140 6 p.

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Isolation and characterization of lactose permease mutants with an enhanced recognition of maltose and diminished recognition of cellobiose.

Collins, J. C., Permuth, S. F. & Brooker, R. J., Sep 5 1989, In: The Journal of biological chemistry. 264, 25, p. 14698-14703 6 p.

Research output: Contribution to journal › Article › peer-review

63 Scopus citations

Isolation and characterization of thiodigalactoside-resistant mutants of the lactose permease which possess an enhanced recognition for maltose

Franco, P. J., Eelkema, J. A. & Brooker, R. J., 1989, In: Journal of Biological Chemistry. 264, 27, p. 15988-15992 5 p.

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

1987

Role of Cysteine Residues in the Lac Permease of Escherichia coli

Menick, D. R., Lee, J. A., Kaback, H. R., Brooker, R. J. & Wilson, T. H., 1987, In: Biochemistry. 26, 4, p. 1132-1136 5 p.

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

1986

Site-specific alteration of cysteine 176 and cysteine 234 in the lactose carrier of Escherichia coli

Brooker, R. J. & Wilson, T. H., 1986, In: Journal of Biological Chemistry. 261, 25, p. 11765-11769 5 p.

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

1985

Characterization of lactose carrier mutants which transport maltose

Brooker, R. J., Fiebig, K. & Wilson, T. H., 1985, In: Journal of Biological Chemistry. 260, 30, p. 16181-16186 6 p.

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Isolation, Characterization, and Nucleotide Sequences of Lactose Permease Mutants That Have Acquired the Ability to Transport Maltose

BROOKER, ROBERT. J. & WILSON, T. HASTINGS., Nov 1985, In: Annals of the New York Academy of Sciences. 456, 1, p. 350 1 p.

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Isolation and nucleotide sequencing of lactose carrier mutants that transport maltose

Brooker, R. J. & Wilson, T. H., 1985, In: Proceedings of the National Academy of Sciences of the United States of America. 82, 12, p. 3959-3963 5 p.

Research output: Contribution to journal › Article › peer-review

Open Access

69 Scopus citations

1984

Electrogenic H⁺ translocation by the plasma membrane ATPase of Neurospora. Studies on plasma membrane vesicles and reconstituted enzyme

Perlin, D. S., Kasamo, K., Brooker, R. J. & Slayman, C. W., 1984, In: Journal of Biological Chemistry. 259, 12, p. 7884-7892 9 p.

Research output: Contribution to journal › Article › peer-review

80 Scopus citations

Mapping, cloning, and DNA sequencing of lac Y mutants

Brooker, R. J. & Wilson, T. H., Jan 1 1984, In: Federation Proceedings. 43, 6, p. no. 1380

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

1983

[14C]N-ethylmaleimide labeling of the plasma membrane [H+]-ATPase of Neurospora crassa.

Brooker, R. J. & Slayman, C. W., Jan 10 1983, In: Journal of Biological Chemistry. 258, 1, p. 222-226 5 p.

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Effects of Mg2+ ions on the plasma membrane [H+]-ATPase of Neurospora crassa. I. Inhibition by N-ethylmaleimide and trypsin.

Brooker, R. J. & Slayman, C. W., Jul 25 1983, In: Journal of Biological Chemistry. 258, 14, p. 8827-8832 6 p.

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Robert J Brooker

Research output

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