TY - JOUR
T1 - α-Viniferin suppresses the signal transducer and activation of transcription-1 (stat-1)-inducible inflammatory genes in interferon-γ-stimulated macrophages
AU - Chung, Eun Yong
AU - Roh, Eunmiri
AU - Kwak, Jeong Ah
AU - Lee, Heun Sik
AU - Lee, Seung Ho
AU - Lee, Chong Kil
AU - Han, Sang Bae
AU - Kim, Youngsoo
PY - 2010/4/16
Y1 - 2010/4/16
N2 - α-Viniferin, an oligostilbene of trimeric resveratrol, has been reported to have anti-inflammatory potential in carrageenin-induced paw edema or adjuvant-induced arthritis in animal models. However, little is known about the molecular basis. In this study, α-viniferin at 3 - 10 μM dose-dependently inhibited interferon (IFN)-γ-induced Ser727 phosphorylation of the signal trans-ducer and activation of transcription-1 (STAT-1), a pivotal transcription factor controlling IFN-γ-targeted genes, in RAW 264.7 macrophages, and also IFN-γ-induced activation of the extracel-lular signal-regulated kinase (ERK)-1, a protein kinase upstream of the Ser727 phosphorylation of STAT-1. However, α-viniferin, only at a higher concentration of 10 μM, inhibited Janus kinase 2-mediated Tyr701 phosphorylation of STAT-1 in the cells. To understand STAT-1-dependent in-flammatory responses, we quantified nitric oxide (NO) or chemokines. α-Viniferin at 3 - 10 μM dose-dependently inhibited IFN-γ-induced production of NO, IFN-γ-inducible protein-10 (IP-10), or the monokine induced by IFN-γ (MIG) in RAW 264.7 cells and also that of NO in primary macrophages-derived from C57BL/6 mice. Furthermore, α-viniferin diminished IFN-γ-induced protein levels of inducible NO synthase (iNOS), attenuated mRNA levels of iNOS, IP-10, or MIG as well as inhibited promoter activity of the iNOS gene. In conclusion, this study proposes an anti-inflammatory mechanism of α-viniferin, down-regulating STAT-1-inducible inflammatory genes via inhibiting ERK-mediated STAT-1 activation in IFN-γ-stimulated macrophages.
AB - α-Viniferin, an oligostilbene of trimeric resveratrol, has been reported to have anti-inflammatory potential in carrageenin-induced paw edema or adjuvant-induced arthritis in animal models. However, little is known about the molecular basis. In this study, α-viniferin at 3 - 10 μM dose-dependently inhibited interferon (IFN)-γ-induced Ser727 phosphorylation of the signal trans-ducer and activation of transcription-1 (STAT-1), a pivotal transcription factor controlling IFN-γ-targeted genes, in RAW 264.7 macrophages, and also IFN-γ-induced activation of the extracel-lular signal-regulated kinase (ERK)-1, a protein kinase upstream of the Ser727 phosphorylation of STAT-1. However, α-viniferin, only at a higher concentration of 10 μM, inhibited Janus kinase 2-mediated Tyr701 phosphorylation of STAT-1 in the cells. To understand STAT-1-dependent in-flammatory responses, we quantified nitric oxide (NO) or chemokines. α-Viniferin at 3 - 10 μM dose-dependently inhibited IFN-γ-induced production of NO, IFN-γ-inducible protein-10 (IP-10), or the monokine induced by IFN-γ (MIG) in RAW 264.7 cells and also that of NO in primary macrophages-derived from C57BL/6 mice. Furthermore, α-viniferin diminished IFN-γ-induced protein levels of inducible NO synthase (iNOS), attenuated mRNA levels of iNOS, IP-10, or MIG as well as inhibited promoter activity of the iNOS gene. In conclusion, this study proposes an anti-inflammatory mechanism of α-viniferin, down-regulating STAT-1-inducible inflammatory genes via inhibiting ERK-mediated STAT-1 activation in IFN-γ-stimulated macrophages.
KW - Anti-inflammation
KW - Interferon (IFN)-γ
KW - Macrophage
KW - Signal transducer and activation of transcription-1 (STAT-1) activation
KW - α-viniferin
UR - http://www.scopus.com/inward/record.url?scp=77951224239&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77951224239&partnerID=8YFLogxK
U2 - 10.1254/jphs.09247FP
DO - 10.1254/jphs.09247FP
M3 - Article
C2 - 20424383
AN - SCOPUS:77951224239
SN - 1347-8613
VL - 112
SP - 405
EP - 414
JO - Journal of Pharmacological Sciences
JF - Journal of Pharmacological Sciences
IS - 4
ER -