μ opioid receptor knockout in mice: Effects on ligand-induced analgesia and morphine lethality

Horace H Loh; Hsien Ching Liu; Antonella Cavalli; Wanling Yang; Yuh Fung Chen; Li-Na Wei

doi:10.1016/S0169-328X(97)00353-7

μ opioid receptor knockout in mice: Effects on ligand-induced analgesia and morphine lethality

Horace H Loh, Hsien Ching Liu, Antonella Cavalli, Wanling Yang, Yuh Fung Chen, Li-Na Wei

Pharmacology

Research output: Contribution to journal › Article › peer-review

301 Scopus citations

Abstract

The μ opioid receptor gene (MOR) was mutated in mice by a gene targeting procedure. In these MOR-knockout mice, the analgesic effects of morphine, its major metabolites, morphine-6-glucuronide (M-6-G) and morphine-6-ethereal sulfate (M-6-S), and endomorphin-2, as well as morphine-induced lethality, were drastically reduced, whereas the effects of DPDPE and U50488 remained unchanged. It is concluded that analgesic effects of μ-specific opioid ligands and acute morphine lethality are mediated by the μ receptor.

Original language	English (US)
Pages (from-to)	321-326
Number of pages	6
Journal	Molecular Brain Research
Volume	54
Issue number	2
DOIs	https://doi.org/10.1016/S0169-328X(97)00353-7
State	Published - Mar 1 1998

Bibliographical note

Funding Information:
We thank Dr. D.W. Melton for granting permission to use the HM1 ES cells, professor Lan Bo Chan for his generous help in many essential parts of this work, Drs. Jim Fujimoto and Pasternak for providing samples of morphine metabolites and Dr. Jim Zadina for providing a sample of endomorphin. This work was supported by NIH grants DA00564, DA01583, DA05695, K05-DA70554, and the F&A Stark Fund of the Minnesota Medical Foundation to HHL.

Keywords

Gene-targeting
Morphine analgesia
Morphine lethality
μ opioid receptor

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abstract = "The μ opioid receptor gene (MOR) was mutated in mice by a gene targeting procedure. In these MOR-knockout mice, the analgesic effects of morphine, its major metabolites, morphine-6-glucuronide (M-6-G) and morphine-6-ethereal sulfate (M-6-S), and endomorphin-2, as well as morphine-induced lethality, were drastically reduced, whereas the effects of DPDPE and U50488 remained unchanged. It is concluded that analgesic effects of μ-specific opioid ligands and acute morphine lethality are mediated by the μ receptor.",

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note = "Funding Information: We thank Dr. D.W. Melton for granting permission to use the HM1 ES cells, professor Lan Bo Chan for his generous help in many essential parts of this work, Drs. Jim Fujimoto and Pasternak for providing samples of morphine metabolites and Dr. Jim Zadina for providing a sample of endomorphin. This work was supported by NIH grants DA00564, DA01583, DA05695, K05-DA70554, and the F&A Stark Fund of the Minnesota Medical Foundation to HHL. ",

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AU - Chen, Yuh Fung

AU - Wei, Li-Na

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AB - The μ opioid receptor gene (MOR) was mutated in mice by a gene targeting procedure. In these MOR-knockout mice, the analgesic effects of morphine, its major metabolites, morphine-6-glucuronide (M-6-G) and morphine-6-ethereal sulfate (M-6-S), and endomorphin-2, as well as morphine-induced lethality, were drastically reduced, whereas the effects of DPDPE and U50488 remained unchanged. It is concluded that analgesic effects of μ-specific opioid ligands and acute morphine lethality are mediated by the μ receptor.

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KW - Morphine analgesia

KW - Morphine lethality

KW - μ opioid receptor

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μ opioid receptor knockout in mice: Effects on ligand-induced analgesia and morphine lethality

Abstract

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