α-Sarcin cleaves ribosomal RNA at the α-sarcin site in the absence of ribosomal proteins

Stephen P Miller, James W. Bodley

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

α-Sarcin is capable of specifically cleaving the single phosphodiester bond in the "α-sarcin site" of both Escherichia coli and Saccharomyces cerevisiae large rRNAs in the absence of ribosomal proteins. With both sources of rRNA, the rate of cleavage was comparable with and without ribosomal proteins but more complete cleavage was observed in the absence of ribosomal proteins. These observations contrast with earlier findings and indicate that ribosomal proteins are not essential to the unique specificity of the cleavage of rRNA by α-sarcin.

Original languageEnglish (US)
Pages (from-to)404-410
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume154
Issue number1
DOIs
StatePublished - Jul 15 1988

Bibliographical note

Funding Information:
ACKNOWLEDGMENTS: This work was supported by grants from NIH (GM-26832), the Graduate School of the University of Minnesota, and the Minnesota Medical Foundation.

Fingerprint Dive into the research topics of 'α-Sarcin cleaves ribosomal RNA at the α-sarcin site in the absence of ribosomal proteins'. Together they form a unique fingerprint.

Cite this