α-Sarcin is capable of specifically cleaving the single phosphodiester bond in the "α-sarcin site" of both Escherichia coli and Saccharomyces cerevisiae large rRNAs in the absence of ribosomal proteins. With both sources of rRNA, the rate of cleavage was comparable with and without ribosomal proteins but more complete cleavage was observed in the absence of ribosomal proteins. These observations contrast with earlier findings and indicate that ribosomal proteins are not essential to the unique specificity of the cleavage of rRNA by α-sarcin.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Jul 15 1988|
Bibliographical noteFunding Information:
ACKNOWLEDGMENTS: This work was supported by grants from NIH (GM-26832), the Graduate School of the University of Minnesota, and the Minnesota Medical Foundation.