TY - JOUR
T1 - A comparative study of an indirect fluorescent antibody test and an immunoperoxidase monolayer assay for the diagnosis of porcine proliferative enteropathy
AU - Guedes, Roberto M.C.
AU - Gebhart, Connie J.
AU - Winkelman, Nathan L.
AU - Mackie-Nuss, Rebecca A.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2002/9
Y1 - 2002/9
N2 - The currently used indirect fluorescent antibody test (IFAT) for the detection of antibodies against porcine proliferative enteropathy (PPE) was compared to an immnnoperoxidase monolayer assay (IPMA). Serum samples used in this comparison were collected from 5-week-old pigs on day O (pre-experimental challenge) and on days 7, 14, 21, and 28 after oral inoculation with intestinal homogenate from pigs affected by PPE (28 challenged pigs) and sucrose phosphate glutamate solution (2 control pigs). All animals were euthanized 4 weeks after inoculation. Immunohistochemistry staining was applied to formalin-fixed, paraffin-embedded sections of ileum for the detection of Lawsonia intracellularis antigen. The serology results with each method agreed in all samples, except on days O and 7 in 1 control animal, which was positive by IPMA, but negative by IFAT. The percentage of agreement between IFAT and IPMA was 98.6%.
AB - The currently used indirect fluorescent antibody test (IFAT) for the detection of antibodies against porcine proliferative enteropathy (PPE) was compared to an immnnoperoxidase monolayer assay (IPMA). Serum samples used in this comparison were collected from 5-week-old pigs on day O (pre-experimental challenge) and on days 7, 14, 21, and 28 after oral inoculation with intestinal homogenate from pigs affected by PPE (28 challenged pigs) and sucrose phosphate glutamate solution (2 control pigs). All animals were euthanized 4 weeks after inoculation. Immunohistochemistry staining was applied to formalin-fixed, paraffin-embedded sections of ileum for the detection of Lawsonia intracellularis antigen. The serology results with each method agreed in all samples, except on days O and 7 in 1 control animal, which was positive by IPMA, but negative by IFAT. The percentage of agreement between IFAT and IPMA was 98.6%.
UR - http://www.scopus.com/inward/record.url?scp=0001498801&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0001498801&partnerID=8YFLogxK
U2 - 10.1177/104063870201400512
DO - 10.1177/104063870201400512
M3 - Review article
C2 - 12296397
AN - SCOPUS:0001498801
SN - 1040-6387
VL - 14
SP - 420
EP - 423
JO - Journal of Veterinary Diagnostic Investigation
JF - Journal of Veterinary Diagnostic Investigation
IS - 5
ER -