A comparative study of procedures for sheep erythrocyte-human-T-lymphocyte rosette formation

Maren L Mahowald, Barry S. Handwerger, Erskine M. Capertone, Steven D. Douglas

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

A comparative study of several published methods for sheep red blood cell-T-lymphocyte rosette formation was performed. Maximum SRBC-rosette formation occurred with AET treated SRBC in medium supplemented with 20% FCS or with untreated SRBC in 100% FCS. Prolongation of the 4°C incubation period from 4 to 18 h enhanced rosette formation. Fluorescein diacetate staining significantly increased calculated percentage of rosette formation. Fluorescein diacetate staining significantly increased calculated percentage of rosette-forming lymphocytes by allowing accurate indentification of the central lymphocytes in morulas.

Original languageEnglish (US)
Pages (from-to)239-245
Number of pages7
JournalJournal of Immunological Methods
Volume15
Issue number3
DOIs
StatePublished - Apr 1977

Bibliographical note

Funding Information:
This work was supported by NIH USPHS Grants AI-12478-01 and HL-06314-15, and by a grant from the Minnesota Arthritis Foundation.

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