TY - JOUR
T1 - A critical assessment of 60 years of maize intragenic recombination
AU - Okagaki, Ron J.
AU - Dukowic-Schulze, Stefanie
AU - Eggleston, William B.
AU - Muehlbauer, Gary J
N1 - Publisher Copyright:
© 2018 Okagaki, Dukowic-Schulze, Eggleston and Muehlbauer.
PY - 2018
Y1 - 2018
N2 - Until the mid-1950s, it was believed that genetic crossovers did not occur within genes. Crossovers occurred between genes, the “beads on a string” model. Then in 1956, Seymour Benzer published his classic paper describing crossing over within a gene, intragenic recombination. This result from a bacteriophage gene prompted Oliver Nelson to study intragenic recombination in the maize Waxy locus. His studies along with subsequent work by others working with maize and other organisms described the outcomes of intragenic recombination and provided some of the earliest evidence that genes, not intergenic regions, were recombination hotspots. High-throughput genotyping approaches have since replaced single gene intragenic studies for characterizing the outcomes of recombination. These large-scale studies confirm that genes, or more generally genic regions, are the most active recombinogenic regions, and suggested a pattern of crossovers similar to the budding yeast Saccharomyces cerevisiae. In S. cerevisiae recombination is initiated by double-strand breaks (DSBs) near transcription start sites (TSSs) of genes producing a polarity gradient where crossovers preferentially resolve at the 5′ end of genes. Intragenic studies in maize yielded less evidence for either polarity or for DSBs near TSSs initiating recombination and in certain respects resembled Schizosaccharomyces pombe or mouse. These different perspectives highlight the need to draw upon the strengths of different approaches and caution against relying on a single model system or approach for understanding recombination.
AB - Until the mid-1950s, it was believed that genetic crossovers did not occur within genes. Crossovers occurred between genes, the “beads on a string” model. Then in 1956, Seymour Benzer published his classic paper describing crossing over within a gene, intragenic recombination. This result from a bacteriophage gene prompted Oliver Nelson to study intragenic recombination in the maize Waxy locus. His studies along with subsequent work by others working with maize and other organisms described the outcomes of intragenic recombination and provided some of the earliest evidence that genes, not intergenic regions, were recombination hotspots. High-throughput genotyping approaches have since replaced single gene intragenic studies for characterizing the outcomes of recombination. These large-scale studies confirm that genes, or more generally genic regions, are the most active recombinogenic regions, and suggested a pattern of crossovers similar to the budding yeast Saccharomyces cerevisiae. In S. cerevisiae recombination is initiated by double-strand breaks (DSBs) near transcription start sites (TSSs) of genes producing a polarity gradient where crossovers preferentially resolve at the 5′ end of genes. Intragenic studies in maize yielded less evidence for either polarity or for DSBs near TSSs initiating recombination and in certain respects resembled Schizosaccharomyces pombe or mouse. These different perspectives highlight the need to draw upon the strengths of different approaches and caution against relying on a single model system or approach for understanding recombination.
KW - Double-strand breaks
KW - Hotspots
KW - Intragenic
KW - Maize
KW - Polarity
KW - Recombination
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U2 - 10.3389/fpls.2018.01560
DO - 10.3389/fpls.2018.01560
M3 - Review article
C2 - 30420864
AN - SCOPUS:85058810059
SN - 1664-462X
VL - 871
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
M1 - 1560
ER -