A distinct pathway of cell-mediated apoptosis initiated by Granulysin

A. A. Kaspar; S. Okada; J. Kumar; F. R. Poulain; K. A. Drouvalakis; A. Kelekar; D. A. Hanson; R. M. Kluck; Y. Hitoshi; D. E. Johnson; C. J. Froelich; C. B. Thompson; D. D. Newmeyer; A. Anel; C. Clayberger; A. M. Krensky

doi:10.4049/jimmunol.167.1.350

A distinct pathway of cell-mediated apoptosis initiated by Granulysin

A. A. Kaspar, S. Okada, J. Kumar, F. R. Poulain, K. A. Drouvalakis, A. Kelekar, D. A. Hanson, R. M. Kluck, Y. Hitoshi, D. E. Johnson, C. J. Froelich, C. B. Thompson, D. D. Newmeyer, A. Anel, C. Clayberger, A. M. Krensky

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

130 Scopus citations

Abstract

Granulysin is an antimicrobial and tumoricidal molecule expressed in granules of CTL and NK cells. In this study, we show that granulysin damages cell membranes based upon negative charge, disrupts the transmembrane potential (Δψ) in mitochondria, and causes release of cytochrome c. Granulysin-induced apoptosis is blocked in cells overexpressing Bcl-2. Despite the release of cytochrome c, procaspase 9 is not processed. Nevertheless, activation of caspase 3 is observed in granulysin-treated cells, suggesting that granulysin activates a novel pathway of CTL- and NK cell-mediated death distinct from granzyme- and death receptor-induced apoptosis.

Original language	English (US)
Pages (from-to)	350-356
Number of pages	7
Journal	Journal of Immunology
Volume	167
Issue number	1
DOIs	https://doi.org/10.4049/jimmunol.167.1.350
State	Published - Jul 1 2001

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Kaspar, A. A., Okada, S., Kumar, J., Poulain, F. R., Drouvalakis, K. A., Kelekar, A., Hanson, D. A., Kluck, R. M., Hitoshi, Y., Johnson, D. E., Froelich, C. J., Thompson, C. B., Newmeyer, D. D., Anel, A., Clayberger, C., & Krensky, A. M. (2001). A distinct pathway of cell-mediated apoptosis initiated by Granulysin. Journal of Immunology, 167(1), 350-356. https://doi.org/10.4049/jimmunol.167.1.350

Kaspar, AA, Okada, S, Kumar, J, Poulain, FR, Drouvalakis, KA, Kelekar, A, Hanson, DA, Kluck, RM, Hitoshi, Y, Johnson, DE, Froelich, CJ, Thompson, CB, Newmeyer, DD, Anel, A, Clayberger, C & Krensky, AM 2001, 'A distinct pathway of cell-mediated apoptosis initiated by Granulysin', Journal of Immunology, vol. 167, no. 1, pp. 350-356. https://doi.org/10.4049/jimmunol.167.1.350

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abstract = "Granulysin is an antimicrobial and tumoricidal molecule expressed in granules of CTL and NK cells. In this study, we show that granulysin damages cell membranes based upon negative charge, disrupts the transmembrane potential (Δψ) in mitochondria, and causes release of cytochrome c. Granulysin-induced apoptosis is blocked in cells overexpressing Bcl-2. Despite the release of cytochrome c, procaspase 9 is not processed. Nevertheless, activation of caspase 3 is observed in granulysin-treated cells, suggesting that granulysin activates a novel pathway of CTL- and NK cell-mediated death distinct from granzyme- and death receptor-induced apoptosis.",

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AU - Kelekar, A.

AU - Hanson, D. A.

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AU - Hitoshi, Y.

AU - Johnson, D. E.

AU - Froelich, C. J.

AU - Thompson, C. B.

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A distinct pathway of cell-mediated apoptosis initiated by Granulysin

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