A Dot Immunobinding Assay on Nitrocellulose for the Detection of Bovid Herpesvirus- Antibodies

Khalid Naeem, Sagar M Goyal

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

A dot immunobinding assay (DIA) was developed for the detection of antibody against bovid herpesvirus-4 (BHV-4) in bovine sera. A semipurified virus preparation was used as the antigen and an anti-species horseradish peroxidase-labeled IgG and diaminobenzidine were employed as the detection system. The sensitivity and specificity of the DIA were similar to those of indirect fluorescent antibody test, indicating the suitability of DIA as a rapid field test for the detection of anti-BHV-4 antibodies in cattle.

Original languageEnglish (US)
Pages (from-to)107-110
Number of pages4
JournalJournal of Veterinary Diagnostic Investigation
Volume2
Issue number2
DOIs
StatePublished - Apr 1990

Bibliographical note

Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.

Fingerprint Dive into the research topics of 'A Dot Immunobinding Assay on Nitrocellulose for the Detection of Bovid Herpesvirus- Antibodies'. Together they form a unique fingerprint.

Cite this