A dual-radioisotope hybrid whole-body micro-positron emission tomography/computed tomography system reveals functional heterogeneity and early local and systemic changes following targeted radiation to the murine caudal skeleton

Masashi Yagi; Luke Arentsen; Ryan M. Shanley; Clifford J. Rosen; Louis S. Kidder; Leslie C. Sharkey; Douglas Yee; Masahiko Koizumi; Kazuhiko Ogawa; Susanta K. Hui

doi:10.1007/s00223-014-9839-6

A dual-radioisotope hybrid whole-body micro-positron emission tomography/computed tomography system reveals functional heterogeneity and early local and systemic changes following targeted radiation to the murine caudal skeleton

Masashi Yagi, Luke Arentsen, Ryan M. Shanley, Clifford J. Rosen, Louis S. Kidder, Leslie C. Sharkey, Douglas Yee, Masahiko Koizumi, Kazuhiko Ogawa, Susanta K. Hui

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The purpose of this study was to develop a longitudinal non-invasive functional imaging method using a dual-radioisotope hybrid micro-positron emission tomography/computed tomography (PET/CT) scanner in order to assess both the skeletal metabolic heterogeneity and the effect of localized radiation that models therapeutic cancer treatment on marrow and bone metabolism. Skeletally mature BALB/c female mice were given clinically relevant local radiation (16 Gy) to the hind limbs on day 0. Micro-PET/CT acquisition was performed serially for the same mice on days -5 and +2 with FDG and days -4 and +3 with NaF. Serum levels of pro-inflammatory cytokines were measured. Significant differences (p < 0.0001) in marrow metabolism (measured by FDG) and bone metabolism (measured by NaF) were observed among bones before radiation, which demonstrates functional heterogeneity in the marrow and mineralized bone throughout the skeleton. Radiation significantly (p < 0.0001) decreased FDG uptake but increased NaF uptake (p = 0.0314) in both irradiated and non-irradiated bones at early time points. An increase in IL-6 was observed with a significant abscopal (distant) effect on marrow and bone metabolic function. Radiation significantly decreased circulating IGF-1 (p < 0.01). Non-invasive longitudinal imaging with dual-radioisotope micro-PET/CT is feasible to investigate simultaneous changes in marrow and bone metabolic function at local and distant skeletal sites in response to focused radiation injury. Distinct local and remote changes may be affected by several cytokines activated early after local radiation exposure. This approach has the potential for longer-term studies to clarify the effects of radiation on marrow and bone.

Original language	English (US)
Pages (from-to)	544-552
Number of pages	9
Journal	Calcified Tissue International
Volume	94
Issue number	5
DOIs	https://doi.org/10.1007/s00223-014-9839-6
State	Published - May 2014

Bibliographical note

Funding Information:
This work was supported by the National Institutes of Health (1R01CA154491-01, 1R03AR055333-01A1, and 1K12-HD055887-01) and the Japan Society for the Promotion of Science Core to Core Program (23003). This work was also supported by PHS Cancer Center Support Grant P30 CA77398 and the Joseph E. Wargo Cancer Research Fund from the University of Minnesota. C. J. R. received funding support from the National Institutes of Health (R24DK092759). The authors acknowledge and thank Dr. Kihak Lee (Siemens Medical Solutions, Knoxville, TN) for advice and fruitful discussion pertaining to this experiment and report, Dr. Bruce E. Hammer (Center for Magnetic Resonance Research, University of Minnesota, Minneapolis, MN) for help with the Siemens micro-PET/CT scanner, and Phuong T. Le (Maine Medical Center Research Institute, Scarborough, ME) for the measurement of pro-inflammatory markers.

Keywords

Bone
Micro-PET/CT
Radiotherapy
Translational research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Yagi, M., Arentsen, L., Shanley, R. M., Rosen, C. J., Kidder, L. S., Sharkey, L. C., Yee, D., Koizumi, M., Ogawa, K., & Hui, S. K. (2014). A dual-radioisotope hybrid whole-body micro-positron emission tomography/computed tomography system reveals functional heterogeneity and early local and systemic changes following targeted radiation to the murine caudal skeleton. Calcified Tissue International, 94(5), 544-552. https://doi.org/10.1007/s00223-014-9839-6

A dual-radioisotope hybrid whole-body micro-positron emission tomography/computed tomography system reveals functional heterogeneity and early local and systemic changes following targeted radiation to the murine caudal skeleton. / Yagi, Masashi; Arentsen, Luke; Shanley, Ryan M. et al.
In: Calcified Tissue International, Vol. 94, No. 5, 05.2014, p. 544-552.

Research output: Contribution to journal › Article › peer-review

Yagi, M, Arentsen, L, Shanley, RM, Rosen, CJ, Kidder, LS, Sharkey, LC, Yee, D, Koizumi, M, Ogawa, K & Hui, SK 2014, 'A dual-radioisotope hybrid whole-body micro-positron emission tomography/computed tomography system reveals functional heterogeneity and early local and systemic changes following targeted radiation to the murine caudal skeleton', Calcified Tissue International, vol. 94, no. 5, pp. 544-552. https://doi.org/10.1007/s00223-014-9839-6

Yagi M, Arentsen L, Shanley RM, Rosen CJ, Kidder LS, Sharkey LC et al. A dual-radioisotope hybrid whole-body micro-positron emission tomography/computed tomography system reveals functional heterogeneity and early local and systemic changes following targeted radiation to the murine caudal skeleton. Calcified Tissue International. 2014 May;94(5):544-552. doi: 10.1007/s00223-014-9839-6

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abstract = "The purpose of this study was to develop a longitudinal non-invasive functional imaging method using a dual-radioisotope hybrid micro-positron emission tomography/computed tomography (PET/CT) scanner in order to assess both the skeletal metabolic heterogeneity and the effect of localized radiation that models therapeutic cancer treatment on marrow and bone metabolism. Skeletally mature BALB/c female mice were given clinically relevant local radiation (16 Gy) to the hind limbs on day 0. Micro-PET/CT acquisition was performed serially for the same mice on days -5 and +2 with FDG and days -4 and +3 with NaF. Serum levels of pro-inflammatory cytokines were measured. Significant differences (p < 0.0001) in marrow metabolism (measured by FDG) and bone metabolism (measured by NaF) were observed among bones before radiation, which demonstrates functional heterogeneity in the marrow and mineralized bone throughout the skeleton. Radiation significantly (p < 0.0001) decreased FDG uptake but increased NaF uptake (p = 0.0314) in both irradiated and non-irradiated bones at early time points. An increase in IL-6 was observed with a significant abscopal (distant) effect on marrow and bone metabolic function. Radiation significantly decreased circulating IGF-1 (p < 0.01). Non-invasive longitudinal imaging with dual-radioisotope micro-PET/CT is feasible to investigate simultaneous changes in marrow and bone metabolic function at local and distant skeletal sites in response to focused radiation injury. Distinct local and remote changes may be affected by several cytokines activated early after local radiation exposure. This approach has the potential for longer-term studies to clarify the effects of radiation on marrow and bone.",

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author = "Masashi Yagi and Luke Arentsen and Shanley, {Ryan M.} and Rosen, {Clifford J.} and Kidder, {Louis S.} and Sharkey, {Leslie C.} and Douglas Yee and Masahiko Koizumi and Kazuhiko Ogawa and Hui, {Susanta K.}",

note = "Funding Information: This work was supported by the National Institutes of Health (1R01CA154491-01, 1R03AR055333-01A1, and 1K12-HD055887-01) and the Japan Society for the Promotion of Science Core to Core Program (23003). This work was also supported by PHS Cancer Center Support Grant P30 CA77398 and the Joseph E. Wargo Cancer Research Fund from the University of Minnesota. C. J. R. received funding support from the National Institutes of Health (R24DK092759). The authors acknowledge and thank Dr. Kihak Lee (Siemens Medical Solutions, Knoxville, TN) for advice and fruitful discussion pertaining to this experiment and report, Dr. Bruce E. Hammer (Center for Magnetic Resonance Research, University of Minnesota, Minneapolis, MN) for help with the Siemens micro-PET/CT scanner, and Phuong T. Le (Maine Medical Center Research Institute, Scarborough, ME) for the measurement of pro-inflammatory markers.",

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AU - Yee, Douglas

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AU - Ogawa, Kazuhiko

AU - Hui, Susanta K.

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A dual-radioisotope hybrid whole-body micro-positron emission tomography/computed tomography system reveals functional heterogeneity and early local and systemic changes following targeted radiation to the murine caudal skeleton

Abstract

Bibliographical note

Keywords

UN SDGs

Access

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