A facile transport assay for H + coupled membrane transport using fluorescence probes

Wanjun Lan, Hongliu Ren, Yu Pang, Chuseng Huang, Yufang Xu, Robert J. Brooker, Jingyan Zhang

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

A facile activity assay for an H +-coupled transporter using florescent probes was developed with an H +-coupled manganese transporter (MntH) as a model. Making use of coupled-proton transport, the transport activity (H +/Mn 2+ cotransport) can be directly determined via fluorescence intensity changes of the probe, 5-(and-6)- carboxyfluorescein (5(6)-FAM). The approach of using highly sensitive fluorescence probes provides a more simple and convenient assay method for the determination of proton-coupled metal-ion uptake by transporters.

Original languageEnglish (US)
Pages (from-to)44-46
Number of pages3
JournalAnalytical Methods
Volume4
Issue number1
DOIs
StatePublished - Jan 1 2012

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