TY - JOUR
T1 - A five-transgene cassette confers broad-spectrum resistance to a fungal rust pathogen in wheat
AU - Luo, Ming
AU - Xie, Liqiong
AU - Chakraborty, Soma
AU - Wang, Aihua
AU - Matny, Oadi
AU - Jugovich, Michelle
AU - Kolmer, James A.
AU - Richardson, Terese
AU - Bhatt, Dhara
AU - Hoque, Mohammad
AU - Patpour, Mehran
AU - Sørensen, Chris
AU - Ortiz, Diana
AU - Dodds, Peter
AU - Steuernagel, Burkhard
AU - Wulff, Brande B.H.
AU - Upadhyaya, Narayana M.
AU - Mago, Rohit
AU - Periyannan, Sambasivam
AU - Lagudah, Evans
AU - Freedman, Roger
AU - Lynne Reuber, T.
AU - Steffenson, Brian J.
AU - Ayliffe, Michael
N1 - Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer Nature America, Inc.
PY - 2021/5
Y1 - 2021/5
N2 - Breeding wheat with durable resistance to the fungal pathogen Puccinia graminis f. sp. tritici (Pgt), a major threat to cereal production, is challenging due to the rapid evolution of pathogen virulence. Increased durability and broad-spectrum resistance can be achieved by introducing more than one resistance gene, but combining numerous unlinked genes by breeding is laborious. Here we generate polygenic Pgt resistance by introducing a transgene cassette of five resistance genes into bread wheat as a single locus and show that at least four of the five genes are functional. These wheat lines are resistant to aggressive and highly virulent Pgt isolates from around the world and show very high levels of resistance in the field. The simple monogenic inheritance of this multigene locus greatly simplifies its use in breeding. However, a new Pgt isolate with virulence to several genes at this locus suggests gene stacks will need strategic deployment to maintain their effectiveness.
AB - Breeding wheat with durable resistance to the fungal pathogen Puccinia graminis f. sp. tritici (Pgt), a major threat to cereal production, is challenging due to the rapid evolution of pathogen virulence. Increased durability and broad-spectrum resistance can be achieved by introducing more than one resistance gene, but combining numerous unlinked genes by breeding is laborious. Here we generate polygenic Pgt resistance by introducing a transgene cassette of five resistance genes into bread wheat as a single locus and show that at least four of the five genes are functional. These wheat lines are resistant to aggressive and highly virulent Pgt isolates from around the world and show very high levels of resistance in the field. The simple monogenic inheritance of this multigene locus greatly simplifies its use in breeding. However, a new Pgt isolate with virulence to several genes at this locus suggests gene stacks will need strategic deployment to maintain their effectiveness.
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U2 - 10.1038/s41587-020-00770-x
DO - 10.1038/s41587-020-00770-x
M3 - Article
C2 - 33398152
AN - SCOPUS:85098802795
SN - 1087-0156
VL - 39
SP - 561
EP - 566
JO - Nature biotechnology
JF - Nature biotechnology
IS - 5
ER -