The preparation and spectroscopic characteristics of a novel coumarin derivative of α-bungarotoxin are described. The suitability of this fluorescent conjugate as a probe of cell surface topography and dynamics of acetylcholine receptors is discussed. Data are presented that show this coumarin-α-bungarotoxin conjugate to be an ideal donor fluorophore for time-resolved resonance energy transfer studies employing fluorescein as an acceptor.
|Original language||English (US)|
|Number of pages||8|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Dec 15 1981|