A general scheme for synthesis of substrate-based polyketide labels for acyl carrier proteins

Erick K. Leggans, David L. Akey, Janet L. Smith, Robert A. Fecik

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

A general strategy to enzymatically label acyl carrier proteins (ACPs) of polyketide synthases has been developed. Incorporation of a chloromethyl ketone or vinyl ketone moiety into polyketide chain elongation intermediate mimics allows for the synthesis of CoA adducts. These CoA adducts undergo enzymatic reaction with Sfp, a phosphopantetheinyl transferase, to afford labeled CurB carrier proteins.

Original languageEnglish (US)
Pages (from-to)5939-5942
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume20
Issue number19
DOIs
StatePublished - Oct 1 2010

Keywords

  • Acyl carrier proteins
  • Biosynthesis
  • Labels
  • Polyketides

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