A hemi-fission intermediate links two mechanistically distinct stages of membrane fission

Juha Pekka Mattila, Anna V. Shnyrova, Anna C. Sundborger, Eva Rodriguez Hortelano, Marc Fuhrmans, Sylvia Neumann, Marcus Müller, Jenny E. Hinshaw, Sandra L. Schmid, Vadim A. Frolov

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58 Scopus citations

Abstract

Fusion and fission drive all vesicular transport. Although topologically opposite, these reactions pass through the same hemi-fusion/fission intermediate, characterized by a âstalkâ in which only the outer membrane monolayers of the two compartments have merged to form a localized non-bilayer connection. Formation of the hemi-fission intermediate requires energy input from proteins catalysing membrane remodelling; however, the relationship between protein conformational rearrangements and hemi-fusion/fission remains obscure. Here we analysed how the GTPase cycle of human dynamin 1, the prototypical membrane fission catalyst, is directly coupled to membrane remodelling. We used intramolecular chemical crosslinking to stabilize dynamin in its GDP·AlF 4 â ' -bound transition state. In the absence of GTP this conformer produced stable hemi-fission, but failed to progress to complete fission, even in the presence of GTP. Further analysis revealed that the pleckstrin homology domain (PHD) locked in its membrane-inserted state facilitated hemi-fission. A second mode of dynamin activity, fuelled by GTP hydrolysis, couples dynamin disassembly with cooperative diminishing of the PHD wedging, thus destabilizing the hemi-fission intermediate to complete fission. Molecular simulations corroborate the bimodal character of dynamin action and indicate radial and axial forces as dominant, although not independent, drivers of hemi-fission and fission transformations, respectively. Mirrored in the fusion reaction, the force bimodality might constitute a general paradigm for leakage-free membrane remodelling.

Original languageEnglish (US)
Pages (from-to)109-113
Number of pages5
JournalNature
Volume524
Issue number7563
DOIs
StatePublished - Aug 6 2015
Externally publishedYes

Bibliographical note

Funding Information:
Acknowledgements We thank J. Chappie for helpful discussions, A. Mohanakrishnan and D. Reed for technical assistance. S.L.S. was supported by National Institutes of Health grant R01-GM42455 and the Welch Foundation Grant I-1823. V.A.F. was supported by grants from the Spanish Ministry of Economy and Competitiveness BFU2012-34885, the Basque Government Program Etortek IE12-332 and European FEDER funds. J.E.H. was supported by the National Institute of Diabetes and Digestive and Kidney Diseases Intramural Research Program. M.F. and M.M. were supported by the Volkswagen foundation and the DFG-CRC803 ‘‘Functionality controlled by organization in and between membranes’’ (B03). Simulations were performed at the Jülich Supercomputing Center and the North-German Supercomputer Alliance Hannover/Berlin. J.-P.M. was supported by a postdoctoral research grant from the Academy of Finland.

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