A High Throughput Method for Generating Dihaploids from Tetraploid Potato

James S. Busse, Shelley H. Jansky, Husain I. Agha, Cari A. Schmitz Carley, Laura M. Shannon, Paul C. Bethke

Research output: Contribution to journalArticlepeer-review

Abstract

There is a worldwide effort to increase the efficiency of potato (Solanum tuberosum L.) cultivar development by using inbred diploid breeding lines. This activity is impeded by the cost and effort required to produce dihaploids from cultivated tetraploid potatoes. We developed a high throughput dihaploid production method based on the 60-year-old method of Peloquin and Hougas. Red Norland inflorescences from commercial fields were transferred to greenhouses. As buds developed, pollen from the dihaploid inducer IVP 101 was applied systematically to thousands of stigmas per trial. Berries were harvested 21 days after pollination. Seeds of putative dihaploids lacking a seed spot marker were retained and ploidy was confirmed using flow cytometry. We recovered 23 dihaploids from 21,651 pollinations. This is a promising method for systematically carrying out thousands of pollinations since the cost of field-grown flowers is dramatically less than that of greenhouse-grown flowers.

Original languageEnglish (US)
Pages (from-to)304-314
Number of pages11
JournalAmerican Journal of Potato Research
Volume98
Issue number4
DOIs
StatePublished - Aug 2021

Bibliographical note

Funding Information:
Authors wish to thank Andy Hamernik for input, expertise and efforts throughout this work. We also thank Laura Vanderploeg (UW-Madison, biochemistry) for photography and image processing efforts. John Jansky (UW-Madison) assisted with pollinations in 2018 and Asma Alkhaja (UW-Madison) helped process seeds in 2018. Rachel Figueroa (UMN-St. Paul) and Nicole Mihelich (UMN-St. Paul) performed flow cytometry analysis. We appreciate the collection, shoot preparation and pollination efforts of many individuals including: Rachel Figueroa, Katelyn Filbrandt, Caroline Hanson,?Jesse Huege, Akpevwe Ikoba, Colin Jones, Alex Knopf, Thomas McGehee, Emily Romdenne, Dakota Schaus, Laura Schulz, Brittany Stokes and Heather Tuttle. Multiple commercial growers assisted in this project including Alsum Farms and Produce, Inc., Friesland, WI; Coloma Farms, Inc., Coloma, WI; Edling Farms, Clear Lake, MN; Ewing Farms Inc., Big Lake, MN; Del Hayes and Sons, Big Lake, MN; Gray Potato Farm, Clear Lake, MN; and Wingard Farms, Elk River, MN. This work was funded by USDA-NIFA award number 2019?51181-30,021 in both states and USDA-NIFA award number 2016?34141-25,707 in Minnesota. This work is dedicated to the loving memory of H. Shirley Busse.

Funding Information:
Authors wish to thank Andy Hamernik for input, expertise and efforts throughout this work. We also thank Laura Vanderploeg (UW-Madison, biochemistry) for photography and image processing efforts. John Jansky (UW-Madison) assisted with pollinations in 2018 and Asma Alkhaja (UW-Madison) helped process seeds in 2018. Rachel Figueroa (UMN-St. Paul) and Nicole Mihelich (UMN-St. Paul) performed flow cytometry analysis. We appreciate the collection, shoot preparation and pollination efforts of many individuals including: Rachel Figueroa, Katelyn Filbrandt, Caroline Hanson, Jesse Huege, Akpevwe Ikoba, Colin Jones, Alex Knopf, Thomas McGehee, Emily Romdenne, Dakota Schaus, Laura Schulz, Brittany Stokes and Heather Tuttle. Multiple commercial growers assisted in this project including Alsum Farms and Produce, Inc., Friesland, WI; Coloma Farms, Inc., Coloma, WI; Edling Farms, Clear Lake, MN; Ewing Farms Inc., Big Lake, MN; Del Hayes and Sons, Big Lake, MN; Gray Potato Farm, Clear Lake, MN; and Wingard Farms, Elk River, MN. This work was funded by USDA-NIFA award number 2019–51181-30,021 in both states and USDA-NIFA award number 2016–34141-25,707 in Minnesota. This work is dedicated to the loving memory of H. Shirley Busse.

Publisher Copyright:
© 2021, This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.

Keywords

  • Diploid potato
  • Ploidy reduction
  • Potato breeding
  • Seed spot marker
  • Solanum phureja IVP 101
  • Solanum tuberosum

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