TY - GEN
T1 - A high-throughput platform to probe modulators of endothelial permeability
AU - Crampton, Alexandra L.
AU - Cummins, Katherine A.
AU - Wood, David K.
PY - 2020/1/1
Y1 - 2020/1/1
N2 - We used collagen microtissues (~200 µm) as a base to grow endothelial monolayers and assess their barrier function in vitro. Using this ECM-based platform, we confirmed endothelial cells adhere and form characteristic intercellular junctions. Using optical sectioning, we measured the endothelial permeability to be 2.76 ± 0.61 x10-8 cm/s. To improve the speed of this functional assessment, we used wide-field imaging to assess endothelial barrier function in a high-throughput manner (n>=471). With this rapid imaging method, we assessed the effect of adding the endothelial coating to the collagen microtissues, finding the change in barrier function to be statistically significant (p<0.01).
AB - We used collagen microtissues (~200 µm) as a base to grow endothelial monolayers and assess their barrier function in vitro. Using this ECM-based platform, we confirmed endothelial cells adhere and form characteristic intercellular junctions. Using optical sectioning, we measured the endothelial permeability to be 2.76 ± 0.61 x10-8 cm/s. To improve the speed of this functional assessment, we used wide-field imaging to assess endothelial barrier function in a high-throughput manner (n>=471). With this rapid imaging method, we assessed the effect of adding the endothelial coating to the collagen microtissues, finding the change in barrier function to be statistically significant (p<0.01).
KW - Collagen
KW - Endothelial permeability
KW - Microfluidic
KW - Microtissue
UR - http://www.scopus.com/inward/record.url?scp=85079694525&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85079694525&partnerID=8YFLogxK
M3 - Conference contribution
T3 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
SP - 997
EP - 998
BT - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PB - Chemical and Biological Microsystems Society
T2 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Y2 - 22 October 2017 through 26 October 2017
ER -