A large volume particulate and water multi-sampler with in situ preservation for microbial and biogeochemical studies

J. A. Breier, Cody S Sheik, D. Gomez-Ibanez, R. T. Sayre-McCord, R. Sanger, C. Rauch, M. Coleman, S. A. Bennett, B. R. Cron, M. Li, C. R. German, Brandy M Toner, G. J. Dick

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


A new tool was developed for large volume sampling to facilitate marine microbiology and biogeochemical studies. It was developed for remotely operated vehicle and hydrocast deployments, and allows for rapid collection of multiple sample types from the water column and dynamic, variable environments such as rising hydrothermal plumes. It was used successfully during a cruise to the hydrothermal vent systems of the Mid-Cayman Rise. The Suspended Particulate Rosette V2 large volume multi-sampling system allows for the collection of 14 sample sets per deployment. Each sample set can include filtered material, whole (unfiltered) water, and filtrate. Suspended particulate can be collected on filters up to 142mm in diameter and pore sizes down to 0.2μm. Filtration is typically at flowrates of 2Lmin-1. For particulate material, filtered volume is constrained only by sampling time and filter capacity, with all sample volumes recorded by digital flowmeter. The suspended particulate filter holders can be filled with preservative and sealed immediately after sample collection. Up to 2L of whole water, filtrate, or a combination of the two, can be collected as part of each sample set. The system is constructed of plastics with titanium fasteners and nickel alloy spring loaded seals. There are no ferrous alloys in the sampling system. Individual sample lines are prefilled with filtered, deionized water prior to deployment and remain sealed unless a sample is actively being collected. This system is intended to facilitate studies concerning the relationship between marine microbiology and ocean biogeochemistry.

Original languageEnglish (US)
Pages (from-to)195-206
Number of pages12
JournalDeep-Sea Research Part I: Oceanographic Research Papers
StatePublished - Dec 1 2014

Bibliographical note

Funding Information:
The SUPR-V2 was funded by the Gordon and Betty Moore Foundation through Grant GBMF2764 to J.B. This work was funded in part by the Gordon and Betty Moore Foundation Grant GBMF2609 to G.D. For field testing we would like to thank the ROV Jason operations team, the R/V Atlantis crew (AT18–16), and the OASES 2012 science party for assistance and support with all aspects of the cruise, which was supported jointly by grants to CRG from the National Science Foundation (NSF; OCE-1061863 ) and National Aeronautics and Space Administration׳s (NASA) Astrobiology Science and Technology for Exploring Planets (ASTEP) program ( NNX09AB75G ) and to M.C. from the NSF ( OCE-1061881 ). The post-cruise contributions of S.A.B. and M.C. were supported through the same ASTEP grant ( NNX09AB75G ) and one of the NSF grants ( OCE-1061881 ) and were carried out at the Jet Propulsion Laboratory (JPL), California Institute of Technology, under contract with the National Aeronautics and Space Administration (NASA). A portion of this research was conducted at beamline of the Advanced Light Source (ALS) and we thank A.L.D. Kilcoyne for research support. The A.L.S. is supported by the Director, Office of Science, Office of Basic Energy Sciences, of the U.S. Department of Energy under Contract no. DE-AC02-05CH11231 . We would like to thank the Microbial Systems Laboratory of the Host Microbiome Initiative of the University of Michigan for sequencing support.

Publisher Copyright:
© 2014 Elsevier Ltd.


  • Deep-sea
  • Geochemistry
  • Hydrothermal vents
  • Instrumentation
  • Microbiology
  • Remotely operated vehicle

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