Gluten strength is an important characteristic, determining the end product quality of durum wheat semolina. To identify the genetic basis of gluten strength in North Dakota durum cultivars, a doubled haploid population was developed from the cross of a weak gluten cultivar 'Rugby' and a strong gluten cultivar 'Maier'. A framework linkage map consisting of 228 markers was constructed and used with phenotypic data on gluten strength (measured by sedimentation volume) to conduct single- and two-locus QTL analyses. Only one consistent QTL (QGs.ndsu-1B) contributing up to 90% of the phenotypic or 93% of the genotypic variation was detected on 1BS. No QTL × QTL or QTL × environment interactions were observed. The QGs.ndsu-1B was flanked by two DArT markers which were converted to STS markers and used along with SSR and EST-SSRs to develop a map of 1BS. QTL analysis delineated QGs.ndsu-1B in a 7.3 cM region flanked by an STS marker (STS-wPt2395) and a SSR marker (wmc85). The adapted background of this material and availability of PCR-based markers closely associated with this locus represent invaluable resources for marker-assisted introgression of gluten strength into other durum wheat varieties. A single QTL segregating in this population also makes it an ideal target for map-based cloning.
Bibliographical noteFunding Information:
This study was funded by the North Dakota Wheat Commission . We would like to thank J. Hegstad, S. Stancyk, Sarah Underdahl, and Sally Mann in addition to the members of the wheat germplasm enhancement and durum breeding projects for their excellent technical assistance.
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- Durum wheat
- Gluten strength
- Marker assisted selection