A Microbiology Teaching Lab: Using Koch's Postulates to Determine the Cause of "peep Pox" in Marshmallow Peeps

John L Dahl, Wayne Gatlin

Research output: Contribution to journalArticlepeer-review

Abstract

Koch's postulates are regularly included in the lecture portion of microbiology courses, but rarely are they demonstrated in a microbiology teaching lab. This is understandable given the logistical challenges of undergraduates working with pathogenic bacteria, ethical concerns using animals, and limited time constraints of a weekly lab period. Here we present a cost-effective, timefriendly lab activity that demonstrates the principles of microbial isolation and infection assays that are part of fulfilling Koch's postulates. The disease is "peep pox" caused by a gelatinase-positive bacterial species hydrolyzing marshmallow peeps that proxy as infected animals.

Original languageEnglish (US)
Pages (from-to)676-679
Number of pages4
JournalAmerican Biology Teacher
Volume80
Issue number9
DOIs
StatePublished - Nov 1 2018

Keywords

  • Bacillus amyloliquefaciens
  • Koch's postulates
  • selective media

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