A microtool for in vitro cell array manipulation

A. Baldi, J. N. Fass, M. N. De Silva, D. J. Odde, B. Ziaie

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports on the fabrication of a transparent micro-post array with supporting pillars for in-vitro cell array manipulation. The capability to manipulate and apply mechanical force to an array of cells will enable the engineering of living neural networks having defined connectivity. The micro-tool described in this paper was designed to achieve this goal. Its transparency permits visual access through a light microscope. When the microtool is placed on top of a cell culture, the tips of the posts are 3-5 μm microns above the surface due to the differential height between the posts and the supporting pillars. This small gap prevents the cells from being damaged when the microtool is translated with respect to the cells, while allowing them to touch and adhere to the posts. The micro-tool was tested by using it to apply force to non-arrayed embryonic chick forebrain cells in vitro. The array was moved at a constant speed of 36 μm/hour during one hour. In response, several neurite-like cytoplasmic processes initiated from cells that had formed adhesions to posts, and these processes elongated in response to further translation. This is the first reported microarray designed to apply mechanical forces to cells.

Original languageEnglish (US)
Title of host publication2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings
EditorsDavid Beebe, Andre Dittmar
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages180-183
Number of pages4
ISBN (Electronic)0780374800, 9780780374805
DOIs
StatePublished - 2002
Event2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Madison, United States
Duration: May 2 2002May 4 2002

Publication series

Name2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings

Other

Other2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology
CountryUnited States
CityMadison
Period5/2/025/4/02

Bibliographical note

Funding Information:
The authors would like to thank the staff of the Microtechnology Laboratory of the University of Minnesota for their assistance. Funding for this project was partially provided by the Spanish Ministry of Science and Technology, NSF-Career Grant No. BES9984995 and NSF-BITS grant No. EIA0130875.

Publisher Copyright:
© 2002 IEEE.

Keywords

  • Micropost
  • cell manipulation
  • neural network

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