A monoclonal anti-peptide antibody recognizes the adrenocorticotropic receptor

B. L. Clarke, K. L. Bost

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

We have produced a monoclonal antibody that specifically recognizes the adrenocorticotropic receptor on rat adrenal cells. The immunogen was designed from an RNA sequence complementary to the mRNA coding for ACTH1-24. This complementary peptide, termed HTCA, has been shown to specifically bind ACTH and was proposed to mimic the ACTH binding site of the hormone receptor. The monoclonal anti-HTCA antibody recognized a restricted domain of the HTCA peptide, bound to Y-1 adrenal cells with a KD of 1.8 nM, and blocked the binding of 125I-ACTH to rat adrenal cells. These findings show that anti-HTCA competes with ACTH for binding to the ACTH receptor.

Original languageEnglish (US)
Pages (from-to)1020-1026
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume168
Issue number3
DOIs
StatePublished - May 16 1990

Bibliographical note

Funding Information:
Acknowledgments. We would like to preparation of this manuscript, Todd assistance, and David Pascual for insightful supported by NIDDKD grant DK 39299.

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