Abstract
With the emergence of high profile fish diseases in the Great Lakes region, surveillance and regulatory inspections of fish populations have increased. This has resulted in a better understanding of known pathogens and isolation of many new pathogens of fish. In this study, a multiplex RT-PCR assay was developed for the detection of three newly discovered fish picornaviruses: bluegill picornavirus-1 (BGPV-1), fathead minnow picornavirus (FHMPV), and eel picornavirus-1 (EPV-1). This assay was found to be very sensitive with a detection limit of 81.9. pg/μl of extracted RNA from a pool of FHMPV and BGPV-1 and was able to detect 501 and 224 gene copies/μl of BGPV-1 and FHMPV, respectively. The assay was highly reproducible and did not cross react with other closely related pathogens. We believe that this new assay provides a rapid and cost effective tool for confirming cell culture isolates and conducting prevalence studies of these newly detected fish picornaviruses.
Original language | English (US) |
---|---|
Pages (from-to) | 131-134 |
Number of pages | 4 |
Journal | Journal of Virological Methods |
Volume | 221 |
DOIs | |
State | Published - 2015 |
Bibliographical note
Publisher Copyright:© 2015 Elsevier B.V..
Keywords
- Bluegill picornavirus
- Eel picornavirus
- Fathead minnow picornavirus
- Multiplex RT-PCR
- Reproducibility
- Sensitivity
- Specificity