Bibliographical noteFunding Information:
The authors are grateful to Dr. R. Will Bouchard for making the slide preparations. Financial support from National Science Foundation (NSF Award #0316503, DEB Division of Environmental Biology) and infrastructural support from the Department of Entomology at the University of Minnesota are acknowledged. This paper is published under the auspices of the Chironomidae Research Group at the University of Minnesota (http://midge.cfans.umn.edu/). The authors thank Dr. Yamamoto, Osaka Prefecture University, Japan and Dr. Yan, Tianjin Normal University, China for providing useful information.