A novel yeast genomic DNA library on a geneticin-resistance vector

Peter A. Jauert; Linnea E. Jensen; David T Kirkpatrick

doi:10.1002/yea.1250

A novel yeast genomic DNA library on a geneticin-resistance vector

Peter A. Jauert, Linnea E. Jensen, David T Kirkpatrick

Biology Teaching and Learning

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Abstract

We have constructed a Saccharomyces cerevisiae genomic library utilizing a novel centromere-containing vector bearing the TRP1 and KANMX4 G418-resistance selectable markers. Random Sau3AI genomic DNA fragments with an average size of 6.4 kb were introduced into a unique BamHI site in the vector's polylinker. Six independent library pools were generated and the characteristics of these pools were determined. This library can be used to introduce low-copy genomic DNA fragments into any strain that is not G418-resistant, making it useful for low-copy overexpression analysis or complementation of mutant phenotypes in strains that lack a suitable auxotrophy marker or only exhibit phenotypes on rich, undefined, growth media.

Original language	English (US)
Pages (from-to)	653-657
Number of pages	5
Journal	Yeast
Volume	22
Issue number	8
DOIs	https://doi.org/10.1002/yea.1250
State	Published - Jun 2005

Keywords

Complementation
Drug Resistance
Library
Overexpression

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AB - We have constructed a Saccharomyces cerevisiae genomic library utilizing a novel centromere-containing vector bearing the TRP1 and KANMX4 G418-resistance selectable markers. Random Sau3AI genomic DNA fragments with an average size of 6.4 kb were introduced into a unique BamHI site in the vector's polylinker. Six independent library pools were generated and the characteristics of these pools were determined. This library can be used to introduce low-copy genomic DNA fragments into any strain that is not G418-resistant, making it useful for low-copy overexpression analysis or complementation of mutant phenotypes in strains that lack a suitable auxotrophy marker or only exhibit phenotypes on rich, undefined, growth media.

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A novel yeast genomic DNA library on a geneticin-resistance vector

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Keywords

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