A Phase I study of RSR13, a radiation-enhancing hemoglobin modifier: Tolerance of repeated intravenous doses and correlation of pharmacokinetics with pharmacodynamics

Brian D. Kavanagh, Shiv R. Khandelwal, Rupert K. Schmidt-Ullrich, John D. Roberts, Edward G. Shaw, Andrew D. Pearlman, Jurgen Venitz, Kathryn E. Dusenbery, Donald J. Abraham, Michael J. Gerber

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34 Scopus citations

Abstract

Purpose: Preclinical studies indicate that RSR13 oxygenates and radiosensitizes hypoxic solid tumors by decreasing the oxygen (O2)-binding affinity of hemoglobin (Hb). A Phase I open-label, multicenter dose and frequency escalation study was conducted to assess the safety, tolerance, pharmacokinetics, and pharmacodynamic effect of daily RSR13 administration to cancer patients receiving concurrent palliative radiotherapy (RT). Methods and Materials: Eligibility criteria included the following: ECOG performance status ≤2; resting and exercise arterial oxygen saturation (SaO2) ≥90%; an indication for palliative RT, 20-40 Gy in 10-15 fractions. RSR13 was administered i.v. via central vein over 60 min immediately before RT. Patients received supplemental O2 via nasal cannula at 4 L/min during RSR13 infusion and RT. Plasma, red blood cell (RBC), and urine RSR13 concentrations were assayed. The pharmacodynamic effect of RSR13 on Hb-O2 binding affinity was quantified by multipoint tonometry and expressed as an increase in p50, defined as the partial pressure of O2 that results in 50% SaO2. The RSR13 dose in the first cohort was 75 mg/kg once a week for two doses; successive cohorts received higher, more frequent doses up to 100 mg/kg/day for 10 days during RT. Results: Twenty patients were enrolled in the study. Repeated daily doses of RSR13 were generally well tolerated. Two adverse events of note occurred: (1) A patient with pre-existing restrictive lung disease had transient persistent hypoxemia after the sixth RSR13 dose; (2) a patient with a recurrent glioma receiving high-dose corticosteroids had edema after the seventh RSR13 dose, likely due to the daily high-volume fluid infusions. Both patients recovered to baseline status with conservative management. Maximum pharmacodynamic effect occurred at the end of RSR13 infusion and was proportional to the RBC RSR13 concentration. After an RSR13 dose of 100 mg/kg, the peak increase in p50 averaged 8.1 mm Hg, consistent with the targeted physiologic effect, and then diminished with a half-life of approximately 5 h. Conclusions: RSR13 was well tolerated in daily doses up to 100 mg/kg administered for 10 days during RT. The combined administration of RSR13 with 4 L/min supplemental O2 yielded pharmacodynamic conditions in which hypoxic tumor radiosensitization can occur. Ongoing Phase II and Phase III studies are evaluating the combination of RT and RSR13 for selected indications, including primary brain tumors, brain metastases, and non-small-cell lung cancer.

Original languageEnglish (US)
Pages (from-to)1133-1139
Number of pages7
JournalInternational Journal of Radiation Oncology Biology Physics
Volume49
Issue number4
DOIs
StatePublished - Mar 15 2001

Bibliographical note

Funding Information:
This work was supported by a grant from Allos Therapeutics, Inc.

Keywords

  • Allosteric modifier
  • Hemoglobin
  • Hypoxia
  • RSR13
  • Radiosensitizer

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