Abstract
We describe the production of single-cycle (sc) and replication-competent recombinant vesicular stomatitis viruses (rcVSVs) displaying heterologous envelope glycoproteins (Envs) on their surface. We prepare scVSVs by transiently expressing HIV-1 Envs or SARS-CoV-2 spike followed by infection of the cells with scVSV particles, which do not carry the vsv-g gene. To prepare rcVSVs, we replace the vsv-g with a specific env-encoding gene, transfect cells with multiple plasmids for production of the genomic RNA and viral proteins, and rescue replication-competent viruses.
Original language | English (US) |
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Article number | 100209 |
Journal | STAR Protocols |
Volume | 1 |
Issue number | 3 |
DOIs | |
State | Published - Dec 18 2020 |
Bibliographical note
Publisher Copyright:© 2020 The Author(s)
Keywords
- Cell culture
- Cell-based assays
- Immunology
- Molecular biology