A radiation hybrid map for the bovine Y Chromosome

Wan Sheng Liu, Paola Mariani, Craig W. Beattie, Leeson J. Alexander, F. Abel Ponce de León

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Screening a bovine Y Chromosome-specific DNA library resulted in 34 new microsatellites, six of which mapped to the pseudoautosomal region (PAR), and 28 localized to the Y-specific region. These microsatellites, together with 23 markers previously mapped to the bovine Y Chr, were scored on a 7000-rad cattle-hamster radiation hybrid (RH) panel. Retention frequency of individual markers ranged from 18.5% to 76.5% with an average of 48.4%. Markers with high retention frequency (>55%) were found to exist in multiple copies on the Y Chr. Thirteen markers were placed on the PAR RH map with the AmelY gene proximal to the pseudoautosomal boundary and 46 markers, including Sry and Tspy gene, on the Y-specific region of the RH map. The microsatellites developed and mapped in this work will be useful for comparative mapping of cattle, sheep, and goat, studying the origin, evolution, and migration of bovidae species and provide an initial platform to develop a high-resolution map of the Y Chr and positional cloning of Y-specific genes.

Original languageEnglish (US)
Pages (from-to)320-326
Number of pages7
JournalMammalian Genome
Volume13
Issue number6
DOIs
StatePublished - Jun 6 2002

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