A rapid and simple procedure for purification of indole-3-acetic acid prior to gc-sim-ms analysis1

Kai Hsien Chen, Anita N. Miller, Glenn W. Patterson, Jerry D. Cohen

Research output: Contribution to journalArticlepeer-review

158 Scopus citations

Abstract

A simple and rapid procedure for the purification of indole-3-acetic acid prior to gas chromatography-selected ion monitoring-mass spectro-graphic analysis was developed using an amino anion exchange minicol-umn and a short high resolution C18 column. Since multiple samples can be prepared at one time, the procedure is more rapid and the sample preparation time is reduced to one-third that normally required. In ad-dition, the final recovery was improved by 40 to 50% over that of a solvent partitioning procedure.

Original languageEnglish (US)
Pages (from-to)822-825
Number of pages4
JournalPlant physiology
Volume86
Issue number3
DOIs
StatePublished - 1988

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