TY - JOUR
T1 - A rapid western blotting protocol for the Xenopus oocyte
AU - Lin-Moshier, Yaping
AU - Marchant, Jonathan S.
PY - 2013/3
Y1 - 2013/3
N2 - Often experimentalists require a quantitative assessment of the levels of heterologously expressed proteins to best interpret changed Ca2+ signaling patterns. Here, we detail a rapid and convenient western blotting method for individual Xenopus oocytes. The method exploits recently introduced rapid blotting systems, commercially available from Invitrogen (iBlot) or Bio-Rad (Trans-Blot Turbo). The key advantage is speed: from live cell to transferred membrane in <1 h. Therefore, oocytes can be conveniently processed for western blotting to assess relative expression levels, even after a long day of Ca2+ imaging experiments.
AB - Often experimentalists require a quantitative assessment of the levels of heterologously expressed proteins to best interpret changed Ca2+ signaling patterns. Here, we detail a rapid and convenient western blotting method for individual Xenopus oocytes. The method exploits recently introduced rapid blotting systems, commercially available from Invitrogen (iBlot) or Bio-Rad (Trans-Blot Turbo). The key advantage is speed: from live cell to transferred membrane in <1 h. Therefore, oocytes can be conveniently processed for western blotting to assess relative expression levels, even after a long day of Ca2+ imaging experiments.
UR - http://www.scopus.com/inward/record.url?scp=84874750701&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84874750701&partnerID=8YFLogxK
U2 - 10.1101/pdb.prot072793
DO - 10.1101/pdb.prot072793
M3 - Article
C2 - 23457341
AN - SCOPUS:84874750701
SN - 1559-6095
VL - 8
SP - 262
EP - 265
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
IS - 3
ER -