A Regulatory Response to Ribosomal Protein Mutations Controls Translation, Growth, and Cell Competition

Chang Hyun Lee, Marianthi Kiparaki, Jorge Blanco, Virginia Folgado, Zhejun Ji, Amit Kumar, Gerard Rimesso, Nicholas E. Baker

Research output: Contribution to journalArticlepeer-review

Abstract

Ribosomes perform protein synthesis but are also involved in signaling processes, the full extent of which are still being uncovered. We report that phenotypes of mutating ribosomal proteins (Rps) are largely due to signaling. Using Drosophila, we discovered that a bZip-domain protein, Xrp1, becomes elevated in Rp mutant cells. Xrp1 reduces translation and growth, delays development, is responsible for gene expression changes, and causes the cell competition of Rp heterozygous cells from genetic mosaics. Without Xrp1, even cells homozygously deleted for Rp genes persist and grow. Xrp1 induction in Rp mutant cells depends on a particular Rp with regulatory effects, RpS12, and precedes overall changes in translation. Thus, effects of Rp mutations, even the reductions in translation and growth, depend on signaling through the Xrp1 pathway and are not simply consequences of reduced ribosome production limiting protein synthesis. One benefit of this system may be to eliminate Rp-mutant cells by cell competition. Certain mutant cells are eliminated only in mosaic tissues. Lee et al. isolate mutations in a bZip-domain protein, Xrp1, that rescue ribosomal protein (Rp) heterozygous mutant cells from such competition. Xrp1 accounts for many aspects of the Rp mutant phenotype, indicating they are not direct consequences of ribosome depletion.

Original languageEnglish (US)
Pages (from-to)456-469.e4
JournalDevelopmental Cell
Volume46
Issue number4
DOIs
StatePublished - Aug 20 2018
Externally publishedYes

Bibliographical note

Funding Information:
The authors thank D. Rio and S. Kurata for strains and reagents; M. Francis, L. Johnston, P. Leopold, D. Rio, J. Warner, and D. Zhang for discussions; and H. Buelow, S. Emmons, D. Pan, and members of our laboratory for comments. This work was supported by a grant from the NIH (GM120451) and by the Albert Einstein College of Medicine Human Genetics Program. Drosophila strains from the Bloomington Drosophila Stock Center (supported by NIH P40OD018537) were used in this study. Confocal Imaging was performed at the Analytical Imaging Facility, Albert Einstein College of Medicine, supported by the NCI cancer center support grant (P30CA013330). This paper includes data from theses partially fulfilling the requirements for the Degree of Doctor of Philosophy in the Graduate Division of Medical Sciences, Albert Einstein College of Medicine, Yeshiva University.

Funding Information:
The authors thank D. Rio and S. Kurata for strains and reagents; M. Francis, L. Johnston, P. Leopold, D. Rio, J. Warner, and D. Zhang for discussions; and H. Buelow, S. Emmons, D. Pan, and members of our laboratory for comments. This work was supported by a grant from the NIH ( GM120451 ) and by the Albert Einstein College of Medicine Human Genetics Program. Drosophila strains from the Bloomington Drosophila Stock Center (supported by NIH P40OD018537 ) were used in this study. Confocal Imaging was performed at the Analytical Imaging Facility, Albert Einstein College of Medicine, supported by the NCI cancer center support grant ( P30CA013330 ). This paper includes data from theses partially fulfilling the requirements for the Degree of Doctor of Philosophy in the Graduate Division of Medical Sciences, Albert Einstein College of Medicine, Yeshiva University.

Publisher Copyright:
© 2018 Elsevier Inc.

Keywords

  • Drosophila development
  • Xrp1
  • cell competition
  • growth regulation
  • minute mutation
  • regulation of translation
  • ribosomal protein
  • ribosomopathy

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