A sensitive assay of transthyretin (prealbumin) in human cerebrospinal fluid in nanogram amounts by ELISA

G. T. Vatassery; H. T. Quach; W. E. Smith; B. A. Benson; John H Eckfeldt

doi:10.1016/0009-8981(91)90344-C

A sensitive assay of transthyretin (prealbumin) in human cerebrospinal fluid in nanogram amounts by ELISA

G. T. Vatassery, H. T. Quach, W. E. Smith, B. A. Benson, John H Eckfeldt

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

A sensitive ELISA method for determining transthyretin (prealbumin) in human cerebrospinal fluid (CSF) is described. The method utilizes goat antihuman transthyretin antibody (IgG fraction) for capture and peroxidase conjugated antibody for color development. The assay has a linear range of 1-4 ng transthyretin added per well. The within-day and between-day coefficients of variation are 5.1 and 6.1%, respectively. The concentration of transthyretin in CSF (ranging from 5 to 20 mg per L) correlated significantly with the corresponding serum concentrations (range 170-420 mg/l). This suggests that synthesis of transthyretin in the brain and peripheral tissues is under similar biological control in normal subjects. The transthyretin concentrations in CSF did not correlate with total CSF protein concentration or age of the subject.

Original language	English (US)
Pages (from-to)	19-25
Number of pages	7
Journal	Clinica Chimica Acta
Volume	197
Issue number	1
DOIs	https://doi.org/10.1016/0009-8981(91)90344-C
State	Published - Feb 28 1991

Bibliographical note

Funding Information:
The investigations were supported by grant AGO6309 from the National Institute of Aging and by research funds from the Department of Veterans Affairs.

Keywords

Cerebrospinal fluid
ELISA
Prealbumin
Transthyretin

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "A sensitive assay of transthyretin (prealbumin) in human cerebrospinal fluid in nanogram amounts by ELISA",

abstract = "A sensitive ELISA method for determining transthyretin (prealbumin) in human cerebrospinal fluid (CSF) is described. The method utilizes goat antihuman transthyretin antibody (IgG fraction) for capture and peroxidase conjugated antibody for color development. The assay has a linear range of 1-4 ng transthyretin added per well. The within-day and between-day coefficients of variation are 5.1 and 6.1%, respectively. The concentration of transthyretin in CSF (ranging from 5 to 20 mg per L) correlated significantly with the corresponding serum concentrations (range 170-420 mg/l). This suggests that synthesis of transthyretin in the brain and peripheral tissues is under similar biological control in normal subjects. The transthyretin concentrations in CSF did not correlate with total CSF protein concentration or age of the subject.",

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AU - Vatassery, G. T.

AU - Quach, H. T.

AU - Smith, W. E.

AU - Benson, B. A.

AU - Eckfeldt, John H

N1 - Funding Information: The investigations were supported by grant AGO6309 from the National Institute of Aging and by research funds from the Department of Veterans Affairs.

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N2 - A sensitive ELISA method for determining transthyretin (prealbumin) in human cerebrospinal fluid (CSF) is described. The method utilizes goat antihuman transthyretin antibody (IgG fraction) for capture and peroxidase conjugated antibody for color development. The assay has a linear range of 1-4 ng transthyretin added per well. The within-day and between-day coefficients of variation are 5.1 and 6.1%, respectively. The concentration of transthyretin in CSF (ranging from 5 to 20 mg per L) correlated significantly with the corresponding serum concentrations (range 170-420 mg/l). This suggests that synthesis of transthyretin in the brain and peripheral tissues is under similar biological control in normal subjects. The transthyretin concentrations in CSF did not correlate with total CSF protein concentration or age of the subject.

AB - A sensitive ELISA method for determining transthyretin (prealbumin) in human cerebrospinal fluid (CSF) is described. The method utilizes goat antihuman transthyretin antibody (IgG fraction) for capture and peroxidase conjugated antibody for color development. The assay has a linear range of 1-4 ng transthyretin added per well. The within-day and between-day coefficients of variation are 5.1 and 6.1%, respectively. The concentration of transthyretin in CSF (ranging from 5 to 20 mg per L) correlated significantly with the corresponding serum concentrations (range 170-420 mg/l). This suggests that synthesis of transthyretin in the brain and peripheral tissues is under similar biological control in normal subjects. The transthyretin concentrations in CSF did not correlate with total CSF protein concentration or age of the subject.

KW - Cerebrospinal fluid

KW - ELISA

KW - Prealbumin

KW - Transthyretin

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A sensitive assay of transthyretin (prealbumin) in human cerebrospinal fluid in nanogram amounts by ELISA

Abstract

Bibliographical note

Keywords

UN SDGs

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