A sex-linked microsatellite locus isolated from the Y chromosome of lake charr, Salvelinus namaycush

Jake Stein, Kent M. Reed, Chris C. Wilson, Ruth B. Phillips

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

A small-insert library was constructed after microdissection of the short arm of the Y chromosome of lake charr, Salvelinus namaycush. Clones from this library were sequenced and two dinucleotide CA-repeat microsatellite sequences were recovered. Oligonucleotide primers for one locus were designed and optimized for amplification by PCR. This locus (designated Yp136) was tested for sex linkage in twelve lake charr families and found to be a distance of 37 centimorgans (cM) from the sex-determining locus. This microsatellite locus was also examined in three informative, gynogenetic/diploid, lake charr crosses for linkage to the centromere on the X chromosome. Results from these families show that this locus is 19 cM from the centromere. The combination of linkage data and microdissection information places the sex-determining locus near the telomere of the Y chromosome in lake charr. This is consistent with studies of several other fish species including some salmonids that place the sex locus near the telomere.

Original languageEnglish (US)
Pages (from-to)211-216
Number of pages6
JournalEnvironmental Biology of Fishes
Volume64
Issue number1-3
DOIs
StatePublished - 2002

Bibliographical note

Funding Information:
degree at the University of Wisconsin-Milwaukee. This research was supported in part by a grant from the USDA NRICGP (93-37205-9171) to KMR and RBP.

Keywords

  • Salmonid
  • Sex locus
  • Telomere

Fingerprint

Dive into the research topics of 'A sex-linked microsatellite locus isolated from the Y chromosome of lake charr, Salvelinus namaycush'. Together they form a unique fingerprint.

Cite this