A method for cryopreserving a 100-μm-thick sheet of tissue produced by cultured rabbit chondrocytes has been developed. The method maintains cell viability and avoids tissue fracture and degradation of mechanical properties. A slow-freeze, fast-thaw procedure with 2 M Me2SO as the cryoprotectant resulted in no tissue fracture and approximately 90% viable cells after storage in culture flasks at -80°C. The cells in the retrieved tissue remained responsive to IL-1β, and tensile and fracture toughness properties of the tissue were not degraded by cryopreservation. (C) 2000 Academic Press.
Bibliographical noteFunding Information:
This work was supported by the SOTA-TEC Fund of the Blandin Foundation and The Catherine Mills Davis Endowment to the Bioengineering Program of the Department of Orthopaedic Surgery.
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