A simple cryopreservation method for the maintenance of cell viability and mechanical integrity of a cultured cartilage analog

Theodore R. Oegema; Laurel B. Deloria; Michelle M. Fedewa; John C. Bischof; Jack L. Lewis

doi:10.1006/cryo.2000.2253

A simple cryopreservation method for the maintenance of cell viability and mechanical integrity of a cultured cartilage analog

Theodore R. Oegema, Laurel B. Deloria, Michelle M. Fedewa, John C. Bischof, Jack L. Lewis

Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

A method for cryopreserving a 100-μm-thick sheet of tissue produced by cultured rabbit chondrocytes has been developed. The method maintains cell viability and avoids tissue fracture and degradation of mechanical properties. A slow-freeze, fast-thaw procedure with 2 M Me₂SO as the cryoprotectant resulted in no tissue fracture and approximately 90% viable cells after storage in culture flasks at -80°C. The cells in the retrieved tissue remained responsive to IL-1β, and tensile and fracture toughness properties of the tissue were not degraded by cryopreservation. (C) 2000 Academic Press.

Original language	English (US)
Pages (from-to)	370-375
Number of pages	6
Journal	Cryobiology
Volume	40
Issue number	4
DOIs	https://doi.org/10.1006/cryo.2000.2253
State	Published - 2000

Bibliographical note

Funding Information:
This work was supported by the SOTA-TEC Fund of the Blandin Foundation and The Catherine Mills Davis Endowment to the Bioengineering Program of the Department of Orthopaedic Surgery.

Keywords

Biology
Biomechanics
Cartilage
Cryoprotection

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title = "A simple cryopreservation method for the maintenance of cell viability and mechanical integrity of a cultured cartilage analog",

abstract = "A method for cryopreserving a 100-μm-thick sheet of tissue produced by cultured rabbit chondrocytes has been developed. The method maintains cell viability and avoids tissue fracture and degradation of mechanical properties. A slow-freeze, fast-thaw procedure with 2 M Me2SO as the cryoprotectant resulted in no tissue fracture and approximately 90% viable cells after storage in culture flasks at -80°C. The cells in the retrieved tissue remained responsive to IL-1β, and tensile and fracture toughness properties of the tissue were not degraded by cryopreservation. (C) 2000 Academic Press.",

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note = "Funding Information: This work was supported by the SOTA-TEC Fund of the Blandin Foundation and The Catherine Mills Davis Endowment to the Bioengineering Program of the Department of Orthopaedic Surgery.",

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AU - Deloria, Laurel B.

AU - Fedewa, Michelle M.

AU - Bischof, John C.

AU - Lewis, Jack L.

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N2 - A method for cryopreserving a 100-μm-thick sheet of tissue produced by cultured rabbit chondrocytes has been developed. The method maintains cell viability and avoids tissue fracture and degradation of mechanical properties. A slow-freeze, fast-thaw procedure with 2 M Me2SO as the cryoprotectant resulted in no tissue fracture and approximately 90% viable cells after storage in culture flasks at -80°C. The cells in the retrieved tissue remained responsive to IL-1β, and tensile and fracture toughness properties of the tissue were not degraded by cryopreservation. (C) 2000 Academic Press.

AB - A method for cryopreserving a 100-μm-thick sheet of tissue produced by cultured rabbit chondrocytes has been developed. The method maintains cell viability and avoids tissue fracture and degradation of mechanical properties. A slow-freeze, fast-thaw procedure with 2 M Me2SO as the cryoprotectant resulted in no tissue fracture and approximately 90% viable cells after storage in culture flasks at -80°C. The cells in the retrieved tissue remained responsive to IL-1β, and tensile and fracture toughness properties of the tissue were not degraded by cryopreservation. (C) 2000 Academic Press.

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KW - Biomechanics

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KW - Cryoprotection

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A simple cryopreservation method for the maintenance of cell viability and mechanical integrity of a cultured cartilage analog

Abstract

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