A simple method of pH control for static and static‐renewal aquatic toxicity tests

In response to the need for pH control in aquatic toxicity tests we developed methods to control pH by manipulating the carbon dioxide concentration in the atmosphere over test solutions We believe the use of CO, is preferable to other types of pH control advantages include less alteration of normal test solution chemistry and use of a natural buffer system to achieve ongoing pH control Physical requirements for the use of this method and currently known limits of its use are described Several methodological variations are discussed, along with example applications of the technique