A single immunization with a recombinant canine adenovirus type 2 expressing the seoul virus Gn glycoprotein confers protective immunity against seoul virus in mice

Zi Guo Yuan; Xiu Ming Li; Yasser Saad Mahmmod; Xiao Hu Wang; Hui Juan Xu; Xiu Xiang Zhang

doi:10.1016/j.vaccine.2009.06.062

A single immunization with a recombinant canine adenovirus type 2 expressing the seoul virus Gn glycoprotein confers protective immunity against seoul virus in mice

Zi Guo Yuan, Xiu Ming Li, Yasser Saad Mahmmod, Xiao Hu Wang, Hui Juan Xu, Xiu Xiang Zhang

Veterinary Population Medicine

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Seoul virus (SEOV), a member of hantavirus genus, is one of the causative agents of hemorrhagic fever with renal syndrome (HFRS) and afflicts tens of thousands of people annually. In this paper, we evaluate the immune response induced by a replication-competent recombinant canine adenovirus type 2 expressing the Gn protein of SEOV (rCAV-2-Gn) in BALB/c mice. Sera from immunized mice contained neutralizing antibodies that could specifically recognize SEOV and neutralize its infectivity in vitro. Moreover, the recombinant virus induced complete protection against a lethal challenge with the highly virulent SEOV strain CC-2. Protective level neutralizing antibodies were maintained for at least 20 weeks. The efficacy of the recombinant was similar to that induced by a currently available inactivated HFRS vaccine. This recombinant virus is therefore a potential alternative to the inactivated vaccine. Crown

Original language	English (US)
Pages (from-to)	5247-5251
Number of pages	5
Journal	Vaccine
Volume	27
Issue number	38
DOIs	https://doi.org/10.1016/j.vaccine.2009.06.062
State	Published - Aug 20 2009

Bibliographical note

Funding Information:
This study was supported by grants from the Program for Changjiang Scholars and Innovative Research Team in University (Grant No. IRT0723).

Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.

Keywords

Canine adenovirus type 2
Gn
Immunity
Mice
Seoul virus

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "A single immunization with a recombinant canine adenovirus type 2 expressing the seoul virus Gn glycoprotein confers protective immunity against seoul virus in mice",

abstract = "Seoul virus (SEOV), a member of hantavirus genus, is one of the causative agents of hemorrhagic fever with renal syndrome (HFRS) and afflicts tens of thousands of people annually. In this paper, we evaluate the immune response induced by a replication-competent recombinant canine adenovirus type 2 expressing the Gn protein of SEOV (rCAV-2-Gn) in BALB/c mice. Sera from immunized mice contained neutralizing antibodies that could specifically recognize SEOV and neutralize its infectivity in vitro. Moreover, the recombinant virus induced complete protection against a lethal challenge with the highly virulent SEOV strain CC-2. Protective level neutralizing antibodies were maintained for at least 20 weeks. The efficacy of the recombinant was similar to that induced by a currently available inactivated HFRS vaccine. This recombinant virus is therefore a potential alternative to the inactivated vaccine. Crown",

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AU - Wang, Xiao Hu

AU - Xu, Hui Juan

AU - Zhang, Xiu Xiang

N1 - Funding Information: This study was supported by grants from the Program for Changjiang Scholars and Innovative Research Team in University (Grant No. IRT0723). Copyright: Copyright 2009 Elsevier B.V., All rights reserved.

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N2 - Seoul virus (SEOV), a member of hantavirus genus, is one of the causative agents of hemorrhagic fever with renal syndrome (HFRS) and afflicts tens of thousands of people annually. In this paper, we evaluate the immune response induced by a replication-competent recombinant canine adenovirus type 2 expressing the Gn protein of SEOV (rCAV-2-Gn) in BALB/c mice. Sera from immunized mice contained neutralizing antibodies that could specifically recognize SEOV and neutralize its infectivity in vitro. Moreover, the recombinant virus induced complete protection against a lethal challenge with the highly virulent SEOV strain CC-2. Protective level neutralizing antibodies were maintained for at least 20 weeks. The efficacy of the recombinant was similar to that induced by a currently available inactivated HFRS vaccine. This recombinant virus is therefore a potential alternative to the inactivated vaccine. Crown

AB - Seoul virus (SEOV), a member of hantavirus genus, is one of the causative agents of hemorrhagic fever with renal syndrome (HFRS) and afflicts tens of thousands of people annually. In this paper, we evaluate the immune response induced by a replication-competent recombinant canine adenovirus type 2 expressing the Gn protein of SEOV (rCAV-2-Gn) in BALB/c mice. Sera from immunized mice contained neutralizing antibodies that could specifically recognize SEOV and neutralize its infectivity in vitro. Moreover, the recombinant virus induced complete protection against a lethal challenge with the highly virulent SEOV strain CC-2. Protective level neutralizing antibodies were maintained for at least 20 weeks. The efficacy of the recombinant was similar to that induced by a currently available inactivated HFRS vaccine. This recombinant virus is therefore a potential alternative to the inactivated vaccine. Crown

KW - Canine adenovirus type 2

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KW - Mice

KW - Seoul virus

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A single immunization with a recombinant canine adenovirus type 2 expressing the seoul virus Gn glycoprotein confers protective immunity against seoul virus in mice

Abstract

Bibliographical note

Keywords

UN SDGs

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