A TaqManR RT-PCR assay for the detection of Feline calicivirus

Yogesh Chander, Ashok K. Tiwari, Suchitra Sajja, M. A. Ramakrishnan, Kay S. Faaberg, Sagar M. Goyal

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Felin calicivirus (FCV) is one of the most common pathogens, of cats causing upper respiratory tract infections. A real time RT-PCR assay was developed for the rapid detection of FCV. A set of probe/primers was designed to amplify a regi on of 151 bp based on the conserved region of 7 FCV strains, (FCV 255, 2280, NADC, F9, KCD, CF I and Urbana) by sequence comparison. The assay was found to be more sensitive than virus isolation and was linear over a wide range of template concentrations.

Original languageEnglish (US)
Pages (from-to)100-106
Number of pages7
JournalInternational Journal of Virology
Volume3
Issue number3
DOIs
StatePublished - 2007

Keywords

  • Feline calicivirus
  • Real time RT-PCR
  • Respiratory infection

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