A TaqManR RT-PCR assay for the detection of Feline calicivirus

Yogesh Chander; Ashok K. Tiwari; Suchitra Sajja; M. A. Ramakrishnan; Kay S. Faaberg; Sagar M. Goyal

doi:10.3923/ijv.2007.100.106

A TaqMan^R RT-PCR assay for the detection of Feline calicivirus

Yogesh Chander, Ashok K. Tiwari, Suchitra Sajja, M. A. Ramakrishnan, Kay S. Faaberg, Sagar M. Goyal

Veterinary Population Medicine

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Felin calicivirus (FCV) is one of the most common pathogens, of cats causing upper respiratory tract infections. A real time RT-PCR assay was developed for the rapid detection of FCV. A set of probe/primers was designed to amplify a regi on of 151 bp based on the conserved region of 7 FCV strains, (FCV 255, 2280, NADC, F9, KCD, CF I and Urbana) by sequence comparison. The assay was found to be more sensitive than virus isolation and was linear over a wide range of template concentrations.

Original language	English (US)
Pages (from-to)	100-106
Number of pages	7
Journal	International Journal of Virology
Volume	3
Issue number	3
DOIs	https://doi.org/10.3923/ijv.2007.100.106
State	Published - 2007

Keywords

Feline calicivirus
Real time RT-PCR
Respiratory infection

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title = "A TaqManR RT-PCR assay for the detection of Feline calicivirus",

abstract = "Felin calicivirus (FCV) is one of the most common pathogens, of cats causing upper respiratory tract infections. A real time RT-PCR assay was developed for the rapid detection of FCV. A set of probe/primers was designed to amplify a regi on of 151 bp based on the conserved region of 7 FCV strains, (FCV 255, 2280, NADC, F9, KCD, CF I and Urbana) by sequence comparison. The assay was found to be more sensitive than virus isolation and was linear over a wide range of template concentrations.",

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AU - Tiwari, Ashok K.

AU - Sajja, Suchitra

AU - Ramakrishnan, M. A.

AU - Faaberg, Kay S.

AU - Goyal, Sagar M.

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AB - Felin calicivirus (FCV) is one of the most common pathogens, of cats causing upper respiratory tract infections. A real time RT-PCR assay was developed for the rapid detection of FCV. A set of probe/primers was designed to amplify a regi on of 151 bp based on the conserved region of 7 FCV strains, (FCV 255, 2280, NADC, F9, KCD, CF I and Urbana) by sequence comparison. The assay was found to be more sensitive than virus isolation and was linear over a wide range of template concentrations.

KW - Feline calicivirus

KW - Real time RT-PCR

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