A terpene synthase is involved in the synthesis of the volatile organic compound sodorifen of Serratia plymuthica 4Rx13

Dajana Domik, Andrea Th�rmer, Teresa Weise, Wolfgang Brandt, Rolf Daniel, Birgit Piechulla

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Bacteria release a plethora of volatile organic compounds, including compounds with extraordinary structures. Sodorifen (IUPAC name: 1,2,4,5,6,7,8-heptamethyl-3-methylenebicyclo[3.2.1]oct-6-ene) is a recently identified and unusual volatile hydrocarbon that is emitted by the rhizobacterium Serratia plymuthica 4R×13. Sodorifen comprises a bicyclic ring structure solely consisting of carbon and hydrogen atoms, where every carbon atom of the skeleton is substituted with either a methyl or a methylene group. This unusual feature of sodorifen made a prediction of its biosynthetic origin very difficult and so far its biosynthesis is unknown. To unravel the biosynthetic pathway we performed genome and transcriptome analyses to identify candidate genes. One knockout mutant (SOD_c20750) showed the desired negative sodorifen phenotype. Here it was shown for the first time that this gene is indispensable for the synthesis of sodorifen and strongly supports the hypothesis that sodorifen descends from the terpene metabolism. SOD_c20750 is the first bacterial terpene cyclase isolated from Serratia spp. and Enterobacteriales. Homology modeling revealed a 3D structure, which exhibits a functional role of amino acids for intermediate cation stabilization (W325) and putative proton acception (Y332). Moreover, the size and hydrophobicity of the active site strongly indicates that indeed the enzyme may catalyze the unusual compound sodorifen.

Original languageEnglish (US)
Article number737
JournalFrontiers in Microbiology
Volume7
Issue numberMAY
DOIs
StatePublished - 2016

Bibliographical note

Funding Information:
The authors thank the Universities of Rostock and G?ttingen and the DFG for financial support. BP was supported by DFG Pi153/28-1. The authors are grateful to Nancy Magnus who generated and tested the following mutants: SOD_c08760, SOD_c10270, SOD_c16920, SOD_c36270, SOD_c36180.

Keywords

  • Bacterial terpene cyclase
  • Serratia
  • Sodorifen
  • Volatile organic compound

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