A three-layer competition-based giant magnetoresistive assay for direct quantification of endoglin from human urine

Balasubramanian Srinivasan; Yuanpeng Li; Ying Jing; Chengguo Xing; Joel Slaton; Jian Ping Wang

doi:10.1021/ac2005229

A three-layer competition-based giant magnetoresistive assay for direct quantification of endoglin from human urine

Balasubramanian Srinivasan, Yuanpeng Li, Ying Jing, Chengguo Xing, Joel Slaton, Jian Ping Wang

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Abstract

This study presents a three-layer competition-based assay for ultrasensitive detection and quantification of endoglin from unprocessed human urine samples using a giant magnetoresistive (GMR) sensor and high-moment magnetic nanoparticle-based biosensing technology. This biosensing platform detects as few as 1000 copies of endoglin at concentrations as low as 83 fM with high detection specificity and has a three-order dynamic range. The results reveal that endoglin levels in urine have the potential to predict for the presence of prostate cancer and to distinguish between prostate cancers of different grades.

Original language	English (US)
Pages (from-to)	2996-3002
Number of pages	7
Journal	Analytical Chemistry
Volume	83
Issue number	8
DOIs	https://doi.org/10.1021/ac2005229
State	Published - Apr 15 2011

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AU - Slaton, Joel

AU - Wang, Jian Ping

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AB - This study presents a three-layer competition-based assay for ultrasensitive detection and quantification of endoglin from unprocessed human urine samples using a giant magnetoresistive (GMR) sensor and high-moment magnetic nanoparticle-based biosensing technology. This biosensing platform detects as few as 1000 copies of endoglin at concentrations as low as 83 fM with high detection specificity and has a three-order dynamic range. The results reveal that endoglin levels in urine have the potential to predict for the presence of prostate cancer and to distinguish between prostate cancers of different grades.

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A three-layer competition-based giant magnetoresistive assay for direct quantification of endoglin from human urine

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