Abstract
A novel time-resolved luminescence biosensor assay for anaplastic lymphoma kinase (ALK) was developed. We used a straightforward strategy to modify a known ALK substrate into a peptide biosensor that can accommodate terbium luminescence sensitization upon its phosphorylation by ALK. Since this strategy is generalizable, this high-throughput screening compatible assay serves as an example for development of other kinase assays that employ terbium luminescence as a read-out.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 362-365 |
| Number of pages | 4 |
| Journal | Chemical Communications |
| Volume | 51 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 7 2015 |
Bibliographical note
Publisher Copyright:© The Royal Society of Chemistry 2015.
