A transcriptionally active, covalently closed minichromosome of cauliflower mosaic virus DNA isolated from infected turnip leaves

Neil Olszewski; Gretchen Hagen; Tom J. Guilfoyle

doi:10.1016/0092-8674(82)90156-8

A transcriptionally active, covalently closed minichromosome of cauliflower mosaic virus DNA isolated from infected turnip leaves

Neil Olszewski, Gretchen Hagen, Tom J. Guilfoyle

Plant and Microbial Biology

Research output: Contribution to journal › Article › peer-review

64 Scopus citations

Abstract

Purified nuclei from turnip leaves infected by cauliflower mosaic virus (CaMV) have been shown to contain a fraction of CaMV DNA that consists of covalently closed circular molecules; possesses a nucleosome structure, based on sensitivity to micrococcal nuclease; and contains nuclear RNA polymerase II that selectively transcribes the coding strand of CaMV DNA in vitro. Our results suggest that the transcriptionally active CaMV DNA is in the form of a minichromosome and that this DNA does not contain the site-specific discontinuities characteristic of the virion.

Original language	English (US)
Pages (from-to)	395-402
Number of pages	8
Journal	Cell
Volume	29
Issue number	2
DOIs	https://doi.org/10.1016/0092-8674(82)90156-8
State	Published - Jun 1982

Access

10.1016/0092-8674(82)90156-8

OpenUrl availability

Full text

Cite this

@article{ca7f583268dd44d98b98cd08a355ca96,

title = "A transcriptionally active, covalently closed minichromosome of cauliflower mosaic virus DNA isolated from infected turnip leaves",

abstract = "Purified nuclei from turnip leaves infected by cauliflower mosaic virus (CaMV) have been shown to contain a fraction of CaMV DNA that consists of covalently closed circular molecules; possesses a nucleosome structure, based on sensitivity to micrococcal nuclease; and contains nuclear RNA polymerase II that selectively transcribes the coding strand of CaMV DNA in vitro. Our results suggest that the transcriptionally active CaMV DNA is in the form of a minichromosome and that this DNA does not contain the site-specific discontinuities characteristic of the virion.",

author = "Neil Olszewski and Gretchen Hagen and Guilfoyle, {Tom J.}",

year = "1982",

month = jun,

doi = "10.1016/0092-8674(82)90156-8",

language = "English (US)",

volume = "29",

pages = "395--402",

journal = "Cell",

issn = "0092-8674",

publisher = "Cell Press",

number = "2",

}

TY - JOUR

T1 - A transcriptionally active, covalently closed minichromosome of cauliflower mosaic virus DNA isolated from infected turnip leaves

AU - Olszewski, Neil

AU - Hagen, Gretchen

AU - Guilfoyle, Tom J.

PY - 1982/6

Y1 - 1982/6

N2 - Purified nuclei from turnip leaves infected by cauliflower mosaic virus (CaMV) have been shown to contain a fraction of CaMV DNA that consists of covalently closed circular molecules; possesses a nucleosome structure, based on sensitivity to micrococcal nuclease; and contains nuclear RNA polymerase II that selectively transcribes the coding strand of CaMV DNA in vitro. Our results suggest that the transcriptionally active CaMV DNA is in the form of a minichromosome and that this DNA does not contain the site-specific discontinuities characteristic of the virion.

AB - Purified nuclei from turnip leaves infected by cauliflower mosaic virus (CaMV) have been shown to contain a fraction of CaMV DNA that consists of covalently closed circular molecules; possesses a nucleosome structure, based on sensitivity to micrococcal nuclease; and contains nuclear RNA polymerase II that selectively transcribes the coding strand of CaMV DNA in vitro. Our results suggest that the transcriptionally active CaMV DNA is in the form of a minichromosome and that this DNA does not contain the site-specific discontinuities characteristic of the virion.

UR - http://www.scopus.com/inward/record.url?scp=0020144756&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020144756&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(82)90156-8

DO - 10.1016/0092-8674(82)90156-8

M3 - Article

C2 - 7116445

AN - SCOPUS:0020144756

SN - 0092-8674

VL - 29

SP - 395

EP - 402

JO - Cell

JF - Cell

IS - 2

ER -

A transcriptionally active, covalently closed minichromosome of cauliflower mosaic virus DNA isolated from infected turnip leaves

Abstract

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this