A unique mechanism for protein processing and degradation in Arabidopsis thaliana

Enrique Rojo, Jan Zouhar, Clay Carter, Valentina Kovaleva, Natasha V. Raikhel

Research output: Contribution to journalArticlepeer-review

118 Scopus citations

Abstract

Precursor protease vesicles are plant-specific compartments containing precursors of enzymes that are thought to participate in the degradation of cellular components in organs undergoing senescence. We report in vivo evidence that the precursor protease vesicle-localized vacuolar processing enzyme-γ (VPEγ) is critical for maturation of the plant vacuolar protease AtCPY. We also provide biochemical and functional evidence that VPEγ is involved in degradation of the vacuolar invertase AtFruct4 in aging tissues. Moreover, a proteomics-based approach identified various proteins found in the vacuoles of aging vpeγ mutants but not in WT plants, suggesting a unique role of VPEγ in protein processing and degradation in Arabidopsis.

Original languageEnglish (US)
Pages (from-to)7389-7394
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number12
DOIs
StatePublished - Jun 10 2003

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