A universal method for the preparation of covalent protein-DNA conjugates for use in creating protein nanostructures

Benjamin P. Duckworth, Yan Chen, James W. Wollack, Yuk Sham, Joachim D. Mueller, T. Andrew Taton, Mark D. Distefano

Research output: Contribution to journalArticlepeer-review

77 Scopus citations

Abstract

(Figure Presented) Protein patterning: A protein, selectively labeled with an azide tag, was reacted with alkyne-functionalized DNA. These protein-DNA conjugates were used to create a DNA-assembled protein nanostructure. This method should facilitate the preparation of a range of protein-DNA hybrid structures with useful structural and functional features.

Original languageEnglish (US)
Pages (from-to)8819-8822
Number of pages4
JournalAngewandte Chemie - International Edition
Volume46
Issue number46
DOIs
StatePublished - 2007

Keywords

  • Click reactions
  • Enzymes
  • Nanostructures
  • Protein labeling
  • Protein structures

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