An expression plasmid (MoPrP.Xho), for use in transgenic mice, was developed from the promoter, 5' intronic, and 3' untranslated sequences of the murine prion protein gene. Analyses of mice harboring the MoPrP.Xho construct with cDNA genes encoding the amyloid precursor protein (APP) and human presenilin I demonstrated that this vector provides relatively high levels of transgene-encoded polypeptides in brains and hearts of transgenic mice. The MoPrP.Xho vector should be very useful in strategies designed to overexpress a variety of wild-type and disease related mutant transgenes in the heart and brain.
Bibliographical noteFunding Information:
Cooper, and Stanley Prusiner for mouse PrP DNA clones and sequencei nformation;D r Gopal Thi-nakaranf or PS1 loop antiserumD; r Allan Levey for monoclonaia ntibodiest o human PS1; Drs Charles WeissmannP,h ilip Wong, George Carlson and Karen Hsiao for helpful discussionsW. e also thank Dustin Englekin,L iesl Awalt, and Luba Romanstevfao r technical assistanceT. his work was supportedb y grants from the NationalI nstituteos f Health in the form of an Alzheimer'sD iseaseR esearchC enter(DLP; 2 P50 Ag 05146)a nd a Leadershipa nd Excellencein Alzheimer's Disease (LEAD) award (DLP and DRB; 1 R35 AG 07914), and grants from the Alzheimer'sD iseasea nd RelatedD isordersA ssociation(D RB), The Kanton of Zurich (MF, CW), SchwiezerischNea tionalfond(sC W), and the Human FontierS cienceP rogram( CW), and by the National Cancer InstituteD, HHS, under contract with ABL (NC and N J). Requestsfo r the vectors hould be addressedto David R. Borchelt.
- Expression vector