A volatile liquid chromatography system for nucleotides

John T. Axelxon; James W. Bodley; Timothy F Walseth

doi:10.1016/0003-2697(81)90371-7

A volatile liquid chromatography system for nucleotides

John T. Axelxon, James W. Bodley, Timothy F Walseth

Pharmacology

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41 Scopus citations

Abstract

A liquid chromatography system useful for the rapid purification of radioactively labeled and nonradioactive ribonucleoside mono-, di-, and triphosphates has been developed. The system employs the strong anion-exchange resin AG MP-1 and low concentrations of the volatile eluting agent trifluoroacetic acid, and is suitable for use with high-performance liquid chromatography systems in either isocratic or gradient elution modes. Most common nucleotides can be completely resolved and nearly quantitatively recovered following solvent volatilization.

Original language	English (US)
Pages (from-to)	357-360
Number of pages	4
Journal	Analytical Biochemistry
Volume	116
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(81)90371-7
State	Published - Sep 15 1981

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A volatile liquid chromatography system for nucleotides

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