Abstract
Gene therapy targeting the spinal cord is an important tool for analyzing mechanisms of nervous system diseases and the development of gene therapies. Analogous to a lumbar puncture in humans, the rodent spinal cord can be accessed through an efficient, noninvasive injection. Here we describe a method for AAV-mediated gene transfer to cells of the spinal cord by intrathecal injection of small quantities of AAV vector.
Original language | English (US) |
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Title of host publication | Methods in Molecular Biology |
Publisher | Humana Press Inc. |
Pages | 199-207 |
Number of pages | 9 |
DOIs | |
State | Published - 2019 |
Publication series
Name | Methods in Molecular Biology |
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Volume | 1950 |
ISSN (Print) | 1064-3745 |
Bibliographical note
Publisher Copyright:© Springer Science+Business Media, LLC, part of Springer Nature 2019.
Keywords
- Adeno-associated
- Dorsal root ganglion
- Spinal cord
- Viral vector