Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography

Christian G. Roessler; Rakhi Agarwal; Marc Allaire; Roberto Alonso-Mori; Babak Andi; José F R Bachega; Martin Bommer; Aaron S. Brewster; Michael C. Browne; Ruchira Chatterjee; Eunsun Cho; Aina E. Cohen; Matthew Cowan; Sammy Datwani; Victor L. Davidson; Jim Defever; Brent Eaton; Richard Ellson; Yiping Feng; Lucien P. Ghislain; James M. Glownia; Guangye Han; Johan Hattne; Julia Hellmich; Annie Héroux; Mohamed Ibrahim; Jan Kern; Anthony Kuczewski; Henrik T. Lemke; Pinghua Liu; Lars Majlof; William M. McClintock; Stuart Myers; Silke Nelsen; Joe Olechno; Allen M. Orville; Nicholas K. Sauter; Alexei S. Soares; S. Michael Soltis; Heng Song; Richard G. Stearns; Rosalie Tran; Yingssu Tsai; Monarin Uervirojnangkoorn; Carrie M. Wilmot; Vittal Yachandra; Junko Yano; Erik T. Yukl; Diling Zhu; Athina Zouni

doi:10.1016/j.str.2016.02.007

Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography

Christian G. Roessler, Rakhi Agarwal, Marc Allaire, Roberto Alonso-Mori, Babak Andi, José F R Bachega, Martin Bommer, Aaron S. Brewster, Michael C. Browne, Ruchira Chatterjee, Eunsun Cho, Aina E. Cohen, Matthew Cowan, Sammy Datwani, Victor L. Davidson, Jim Defever, Brent Eaton, Richard Ellson, Yiping Feng, Lucien P. GhislainJames M. Glownia, Guangye Han, Johan Hattne, Julia Hellmich, Annie Héroux, Mohamed Ibrahim, Jan Kern, Anthony Kuczewski, Henrik T. Lemke, Pinghua Liu, Lars Majlof, William M. McClintock, Stuart Myers, Silke Nelsen, Joe Olechno, Allen M. Orville, Nicholas K. Sauter, Alexei S. Soares, S. Michael Soltis, Heng Song, Richard G. Stearns, Rosalie Tran, Yingssu Tsai, Monarin Uervirojnangkoorn, Carrie M. Wilmot, Vittal Yachandra, Junko Yano, Erik T. Yukl, Diling Zhu, Athina Zouni

Biochemistry, Molecular Biology, and Biophysics (CBS)

Research output: Contribution to journal › Article › peer-review

87 Scopus citations

Abstract

X-ray free-electron lasers (XFELs) provide very intense X-ray pulses suitable for macromolecular crystallography. Each X-ray pulse typically lasts for tens of femtoseconds and the interval between pulses is many orders of magnitude longer. Here we describe two novel acoustic injection systems that use focused sound waves to eject picoliter to nanoliter crystal-containing droplets out of microplates and into the X-ray pulse from which diffraction data are collected. The on-demand droplet delivery is synchronized to the XFEL pulse scheme, resulting in X-ray pulses intersecting up to 88% of the droplets. We tested several types of samples in a range of crystallization conditions, wherein the overall crystal hit ratio (e.g., fraction of images with observable diffraction patterns) is a function of the microcrystal slurry concentration. We report crystal structures from lysozyme, thermolysin, and stachydrine demethylase (Stc2). Additional samples were screened to demonstrate that these methods can be applied to rare samples.

Original language	English (US)
Pages (from-to)	631-640
Number of pages	10
Journal	Structure
Volume	24
Issue number	4
DOIs	https://doi.org/10.1016/j.str.2016.02.007
State	Published - Apr 5 2016

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© 2016 Elsevier Ltd. All rights reserved.

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Roessler, C. G., Agarwal, R., Allaire, M., Alonso-Mori, R., Andi, B., Bachega, J. F. R., Bommer, M., Brewster, A. S., Browne, M. C., Chatterjee, R., Cho, E., Cohen, A. E., Cowan, M., Datwani, S., Davidson, V. L., Defever, J., Eaton, B., Ellson, R., Feng, Y., ... Zouni, A. (2016). Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography. Structure, 24(4), 631-640. https://doi.org/10.1016/j.str.2016.02.007

Roessler, CG, Agarwal, R, Allaire, M, Alonso-Mori, R, Andi, B, Bachega, JFR, Bommer, M, Brewster, AS, Browne, MC, Chatterjee, R, Cho, E, Cohen, AE, Cowan, M, Datwani, S, Davidson, VL, Defever, J, Eaton, B, Ellson, R, Feng, Y, Ghislain, LP, Glownia, JM, Han, G, Hattne, J, Hellmich, J, Héroux, A, Ibrahim, M, Kern, J, Kuczewski, A, Lemke, HT, Liu, P, Majlof, L, McClintock, WM, Myers, S, Nelsen, S, Olechno, J, Orville, AM, Sauter, NK, Soares, AS, Soltis, SM, Song, H, Stearns, RG, Tran, R, Tsai, Y, Uervirojnangkoorn, M, Wilmot, CM, Yachandra, V, Yano, J, Yukl, ET, Zhu, D & Zouni, A 2016, 'Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography', Structure, vol. 24, no. 4, pp. 631-640. https://doi.org/10.1016/j.str.2016.02.007

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title = "Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography",

abstract = "X-ray free-electron lasers (XFELs) provide very intense X-ray pulses suitable for macromolecular crystallography. Each X-ray pulse typically lasts for tens of femtoseconds and the interval between pulses is many orders of magnitude longer. Here we describe two novel acoustic injection systems that use focused sound waves to eject picoliter to nanoliter crystal-containing droplets out of microplates and into the X-ray pulse from which diffraction data are collected. The on-demand droplet delivery is synchronized to the XFEL pulse scheme, resulting in X-ray pulses intersecting up to 88% of the droplets. We tested several types of samples in a range of crystallization conditions, wherein the overall crystal hit ratio (e.g., fraction of images with observable diffraction patterns) is a function of the microcrystal slurry concentration. We report crystal structures from lysozyme, thermolysin, and stachydrine demethylase (Stc2). Additional samples were screened to demonstrate that these methods can be applied to rare samples.",

author = "Roessler, {Christian G.} and Rakhi Agarwal and Marc Allaire and Roberto Alonso-Mori and Babak Andi and Bachega, {Jos{\'e} F R} and Martin Bommer and Brewster, {Aaron S.} and Browne, {Michael C.} and Ruchira Chatterjee and Eunsun Cho and Cohen, {Aina E.} and Matthew Cowan and Sammy Datwani and Davidson, {Victor L.} and Jim Defever and Brent Eaton and Richard Ellson and Yiping Feng and Ghislain, {Lucien P.} and Glownia, {James M.} and Guangye Han and Johan Hattne and Julia Hellmich and Annie H{\'e}roux and Mohamed Ibrahim and Jan Kern and Anthony Kuczewski and Lemke, {Henrik T.} and Pinghua Liu and Lars Majlof and McClintock, {William M.} and Stuart Myers and Silke Nelsen and Joe Olechno and Orville, {Allen M.} and Sauter, {Nicholas K.} and Soares, {Alexei S.} and Soltis, {S. Michael} and Heng Song and Stearns, {Richard G.} and Rosalie Tran and Yingssu Tsai and Monarin Uervirojnangkoorn and Wilmot, {Carrie M.} and Vittal Yachandra and Junko Yano and Yukl, {Erik T.} and Diling Zhu and Athina Zouni",

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AU - Roessler, Christian G.

AU - Agarwal, Rakhi

AU - Allaire, Marc

AU - Alonso-Mori, Roberto

AU - Andi, Babak

AU - Bachega, José F R

AU - Bommer, Martin

AU - Brewster, Aaron S.

AU - Browne, Michael C.

AU - Chatterjee, Ruchira

AU - Cho, Eunsun

AU - Cohen, Aina E.

AU - Cowan, Matthew

AU - Datwani, Sammy

AU - Davidson, Victor L.

AU - Defever, Jim

AU - Eaton, Brent

AU - Ellson, Richard

AU - Feng, Yiping

AU - Ghislain, Lucien P.

AU - Glownia, James M.

AU - Han, Guangye

AU - Hattne, Johan

AU - Hellmich, Julia

AU - Héroux, Annie

AU - Ibrahim, Mohamed

AU - Kern, Jan

AU - Kuczewski, Anthony

AU - Lemke, Henrik T.

AU - Liu, Pinghua

AU - Majlof, Lars

AU - McClintock, William M.

AU - Myers, Stuart

AU - Nelsen, Silke

AU - Olechno, Joe

AU - Orville, Allen M.

AU - Sauter, Nicholas K.

AU - Soares, Alexei S.

AU - Soltis, S. Michael

AU - Song, Heng

AU - Stearns, Richard G.

AU - Tran, Rosalie

AU - Tsai, Yingssu

AU - Uervirojnangkoorn, Monarin

AU - Wilmot, Carrie M.

AU - Yachandra, Vittal

AU - Yano, Junko

AU - Yukl, Erik T.

AU - Zhu, Diling

AU - Zouni, Athina

PY - 2016/4/5

Y1 - 2016/4/5

N2 - X-ray free-electron lasers (XFELs) provide very intense X-ray pulses suitable for macromolecular crystallography. Each X-ray pulse typically lasts for tens of femtoseconds and the interval between pulses is many orders of magnitude longer. Here we describe two novel acoustic injection systems that use focused sound waves to eject picoliter to nanoliter crystal-containing droplets out of microplates and into the X-ray pulse from which diffraction data are collected. The on-demand droplet delivery is synchronized to the XFEL pulse scheme, resulting in X-ray pulses intersecting up to 88% of the droplets. We tested several types of samples in a range of crystallization conditions, wherein the overall crystal hit ratio (e.g., fraction of images with observable diffraction patterns) is a function of the microcrystal slurry concentration. We report crystal structures from lysozyme, thermolysin, and stachydrine demethylase (Stc2). Additional samples were screened to demonstrate that these methods can be applied to rare samples.

AB - X-ray free-electron lasers (XFELs) provide very intense X-ray pulses suitable for macromolecular crystallography. Each X-ray pulse typically lasts for tens of femtoseconds and the interval between pulses is many orders of magnitude longer. Here we describe two novel acoustic injection systems that use focused sound waves to eject picoliter to nanoliter crystal-containing droplets out of microplates and into the X-ray pulse from which diffraction data are collected. The on-demand droplet delivery is synchronized to the XFEL pulse scheme, resulting in X-ray pulses intersecting up to 88% of the droplets. We tested several types of samples in a range of crystallization conditions, wherein the overall crystal hit ratio (e.g., fraction of images with observable diffraction patterns) is a function of the microcrystal slurry concentration. We report crystal structures from lysozyme, thermolysin, and stachydrine demethylase (Stc2). Additional samples were screened to demonstrate that these methods can be applied to rare samples.

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JO - Structure

JF - Structure

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ER -

Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography

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