Actin turnover maintains actin filament homeostasis during cytokinetic ring contraction

Ting Gang Chew, Junqi Huang, Saravanan Palani, Ruth Sommese, Anton Kamnev, Tomoyuki Hatano, Ying Gu, Snezhana Oliferenko, Sivaraj Sivaramakrishnan, Mohan K. Balasubramanian

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Cytokinesis in many eukaryotes involves a tension-generating actomyosin-based contractile ring. Many components of actomyosin rings turn over during contraction, although the significance of this turnover has remained enigmatic. Here, using Schizosaccharomyces japonicus, we investigate the role of turnover of actin and myosin II in its contraction. Actomyosin ring components self-organize into ~1-μm-spaced clusters instead of undergoing full-ring contraction in the absence of continuous actin polymerization. This effect is reversed when actin filaments are stabilized. We tested the idea that the function of turnover is to ensure actin filament homeostasis in a synthetic system, in which we abolished turnover by fixing rings in cell ghosts with formaldehyde. We found that these rings contracted fully upon exogenous addition of a vertebrate myosin. We conclude that actin turnover is required to maintain actin filament homeostasis during ring contraction and that the requirement for turnover can be bypassed if homeostasis is achieved artificially.

Original languageEnglish (US)
Pages (from-to)2657-2667
Number of pages11
JournalJournal of Cell Biology
Volume216
Issue number9
DOIs
StatePublished - Sep 1 2017

Bibliographical note

Funding Information:
This work was supported by Wellcome Trust Senior Investigator Awards to M.K. Balasubramanian (WT101885MA) and S. Olife-renko (103741/Z/14/Z). For this work, M.K. Balasubramanian was also supported by a Royal Society Wolfson Merit Award and an European Research Council Advanced Grant (ERC-2014-ADG no. 671083). The authors declare no competing financial interests.

Publisher Copyright:
© 2017 Chew et al.

Fingerprint

Dive into the research topics of 'Actin turnover maintains actin filament homeostasis during cytokinetic ring contraction'. Together they form a unique fingerprint.

Cite this