TY - JOUR
T1 - Activated human monocytes express the c-sis proto-oncogene and release a mediator showing PDGF-like activity
AU - Martinet, Yves
AU - Bitterman, Peter B.
AU - Mornex, Jean Francois
AU - Grotendorst, Gary R.
AU - Martin, George R.
AU - Crystal, Ronald G.
PY - 1986/12/1
Y1 - 1986/12/1
N2 - Current ideas about the mechanism of wound healing and the pathogenesis of atherosclerosis, pulmonary fibrosis and hepatic fibrosis suggest a central role for the mononuclear phagocyte in attracting and/or stimulating the proliferation of mesenchymal cells1-5. We demonstrate here that activated human blood monocytes, but not resting monocytes, release a mediator that attracts smooth muscle cells and cooperates with other mediators to stimulate fibroblast proliferation. This mediator is very similar to platelet-derived growth factor (PDGF): its Chromatographic properties and chemical stability are similar to those of PDGF6,7, it competes with 125I-PDGF for binding to fibroblasts and it immunoprecipitates with anti-PDGF antibodies. In parallel, stimulated monocytes, but not resting monocytes, express the c-sis proto-oncogene, a gene coding for one of the PDGF chains8,9, consistent with the concept that expression of the c-sis proto-oncogene may be involved in the ability of mononuclear phagocytes to modulate the accumulation of mesenchymal cells.
AB - Current ideas about the mechanism of wound healing and the pathogenesis of atherosclerosis, pulmonary fibrosis and hepatic fibrosis suggest a central role for the mononuclear phagocyte in attracting and/or stimulating the proliferation of mesenchymal cells1-5. We demonstrate here that activated human blood monocytes, but not resting monocytes, release a mediator that attracts smooth muscle cells and cooperates with other mediators to stimulate fibroblast proliferation. This mediator is very similar to platelet-derived growth factor (PDGF): its Chromatographic properties and chemical stability are similar to those of PDGF6,7, it competes with 125I-PDGF for binding to fibroblasts and it immunoprecipitates with anti-PDGF antibodies. In parallel, stimulated monocytes, but not resting monocytes, express the c-sis proto-oncogene, a gene coding for one of the PDGF chains8,9, consistent with the concept that expression of the c-sis proto-oncogene may be involved in the ability of mononuclear phagocytes to modulate the accumulation of mesenchymal cells.
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U2 - 10.1038/319158a0
DO - 10.1038/319158a0
M3 - Article
C2 - 3941744
AN - SCOPUS:0022647318
SN - 0028-0836
VL - 319
SP - 158
EP - 160
JO - Nature
JF - Nature
IS - 6049
ER -