Activation of antigen-specific T cells by artificial cell constructs having immobilized multimeric peptide-class I complexes and recombinant B7-Fc proteins

Ee Loon Tham; Paige L. Jensen; Matthew F. Mescher

doi:10.1016/S0022-1759(00)00335-5

Activation of antigen-specific T cells by artificial cell constructs having immobilized multimeric peptide-class I complexes and recombinant B7-Fc proteins

Ee Loon Tham, Paige L. Jensen, Matthew F. Mescher

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

T cell activation results from the engagement of multiple receptors on T cells by their respective ligands on antigen presenting cells. Studies using artificial cell surface constructs have demonstrated that effective T cell response requires that antigen be presented on a solid surface with dimensions that approximate those of an intact cell. In this report, we describe the cloning and expression of recombinant B7-1-Fc and B7-2-Fc proteins and their incorporation onto 5-μm latex microspheres along with renatured peptide-MHC. These microspheres provide a simple and effective method for the in vitro or in vivo stimulation of antigen-specific T cells under precisely controlled antigen and costimulation conditions.

Original language	English (US)
Pages (from-to)	111-119
Number of pages	9
Journal	Journal of Immunological Methods
Volume	249
Issue number	1-2
DOIs	https://doi.org/10.1016/S0022-1759(00)00335-5
State	Published - Mar 1 2001

Bibliographical note

Funding Information:
This work was supported by grants AI 35296, AI 34824 and AI 31524 from the National Institutes of Health. The authors thank John Altman and Eric Pamer for plasmids and protocols, and Mark A. Daniels, Jodi E. Goldberg and Paul L. Weaver for preparing the H-2K b –OVA complexes. We would also like to thank the following R&D Systems, Inc. employees for their contributions to this work: Monica Tsang, Li Zhou, Richard Krzyzek, John Humphrey, Greg Fransen, Boling Zheng, Jie Li, Kevin VanGheem and Marnelle Andersen.

Keywords

Artificial cell surface constructs
Costimulation
Lymphocyte activation

UN SDGs

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title = "Activation of antigen-specific T cells by artificial cell constructs having immobilized multimeric peptide-class I complexes and recombinant B7-Fc proteins",

abstract = "T cell activation results from the engagement of multiple receptors on T cells by their respective ligands on antigen presenting cells. Studies using artificial cell surface constructs have demonstrated that effective T cell response requires that antigen be presented on a solid surface with dimensions that approximate those of an intact cell. In this report, we describe the cloning and expression of recombinant B7-1-Fc and B7-2-Fc proteins and their incorporation onto 5-μm latex microspheres along with renatured peptide-MHC. These microspheres provide a simple and effective method for the in vitro or in vivo stimulation of antigen-specific T cells under precisely controlled antigen and costimulation conditions.",

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KW - Artificial cell surface constructs

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Activation of antigen-specific T cells by artificial cell constructs having immobilized multimeric peptide-class I complexes and recombinant B7-Fc proteins

Abstract

Bibliographical note

Keywords

UN SDGs

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