T cell activation results from the engagement of multiple receptors on T cells by their respective ligands on antigen presenting cells. Studies using artificial cell surface constructs have demonstrated that effective T cell response requires that antigen be presented on a solid surface with dimensions that approximate those of an intact cell. In this report, we describe the cloning and expression of recombinant B7-1-Fc and B7-2-Fc proteins and their incorporation onto 5-μm latex microspheres along with renatured peptide-MHC. These microspheres provide a simple and effective method for the in vitro or in vivo stimulation of antigen-specific T cells under precisely controlled antigen and costimulation conditions.
Bibliographical noteFunding Information:
This work was supported by grants AI 35296, AI 34824 and AI 31524 from the National Institutes of Health. The authors thank John Altman and Eric Pamer for plasmids and protocols, and Mark A. Daniels, Jodi E. Goldberg and Paul L. Weaver for preparing the H-2K b –OVA complexes. We would also like to thank the following R&D Systems, Inc. employees for their contributions to this work: Monica Tsang, Li Zhou, Richard Krzyzek, John Humphrey, Greg Fransen, Boling Zheng, Jie Li, Kevin VanGheem and Marnelle Andersen.
- Artificial cell surface constructs
- Lymphocyte activation